高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (12): 2168-2175.doi: 10.7503/cjcu20160500

• 有机化学 • 上一篇    下一篇

黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、 结构分析及体外抗氧化作用

刘磊1,2,3, 李科2,3(), 郝霞1,2,3, 王桂臻1,2,3, 秦雪梅2,3, 杜冠华4, 张翔5   

  1. 1. 山西大学化学化工学院, 2. 中医药现代研究中心, 太原 030006
    3. 中药功效物质与新药创制山西省重点实验室, 太原 030006
    4. 中国医学科学院、 中国协和医科大学药物研究所, 北京 100050
    5. 美国路易斯维尔大学, 路易斯维尔 40201
  • 收稿日期:2016-07-13 出版日期:2016-12-10 发布日期:2016-11-11
  • 作者简介:联系人简介: 李 科, 男, 博士, 副教授, 主要从事糖化学与糖生物学方面的研究. E-mail:like@sxu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 31300278)、 山西省科技攻关计划(批准号: 20150313004-5)和教育部博士点新教师类基金(20131401120006)资助

Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root

LIU Lei1,2,3, LI Ke2,3,*(), HAO Xia1,2,3, WANG Guizhen1,2,3, QIN Xuemei2,3, DU Guanhua4, ZHANG Xiang5   

  1. 1. College of Chemistry and Chemical Engineering
    2. Modern Research Center for Traditional Chinese Medicine,Shanxi University, Taiyuan 030006, China
    3. The Efficacy of Chinese Medicine Material and New Drug Key Lab of Shanxi Province, Taiyuan 030006, China
    4. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
    5. Medical College, University of Louisville, Louisville 40201, USA
  • Received:2016-07-13 Online:2016-12-10 Published:2016-11-11
  • Contact: LI Ke E-mail:like@sxu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.31300278), the Science and Technology Research Program Fund for Shanxi Province, China(No.20150313004-5) and the Ministry of Education PhD New Teacher Fund, China(No.20131401120006)

摘要:

黄芪药渣经分级提取, 再通过二乙氨乙基(DEAE)-纤维素52阴离子交换柱层析和Sephacryl S-400 HR凝胶柱层析分离纯化, 得到4个多糖组分AX-Ⅰ-1~AX-Ⅰ-4. 对多糖AX-Ⅰ-1的理化性质和结构进行研究发现, AX-Ⅰ-1含有鼠李糖、 阿拉伯糖、 木糖、 甘露糖、 葡萄糖和半乳糖(摩尔比为0.006∶14.113∶8.284∶0.116∶0.468∶1), 主链由阿拉伯糖和木糖通过β-(1→2), (1→3)和(1→4)苷键组成, 支链由(1→4)βArap, (1→3)βGalp和(1→2)βMan组成, 非还原末端由αRhap, βGclpβGalp组成. 对多糖AX-Ⅰ-1~AX-Ⅰ-4的抗氧化研究结果表明, 这4个组分对羟基自由基、 超氧阴离子自由基和1,1-二苯基-2-三硝基苯肼(DPPH)自由基均有一定的清除作用; 当浓度为0.1 mg/mL时, 多糖AX-Ⅰ-2~AX-Ⅰ-4对超氧阴离子的清除率是阳性对照维生素C(Vc)的2倍.

关键词: 黄芪药渣, 阿拉伯木聚糖, 分离纯化, 结构分析, 抗氧化

Abstract:

Four fractions(AX-Ⅰ-1, AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4)from the residue of Astragalus root were extracted by sequential chemical extraction, and purified by DEAE-Cellulose 52 anion-exchange and Sepharcryl S-400 high resolution gel-permeation chromatography. A combination of chemical and instrumental analysis was performed to investigate the structural characterization and antioxidant activity of AX-Ⅰ-1. The results demonstrated that AX-Ⅰ-1 was mainly composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, and the molar ratio was 0.006∶14.113∶8.284∶0.116∶0.468∶1. Backbone of AX-Ⅰ-1 composed of arabinose and xylose by β-(1→2), β-(1→3)and β-(1→4)glycosidic bond. The branches were composed of (1→4)-linkedArap, (1→3)-linkedGalp, (1→2)-linkedManp, and the terminal residues were αRhap, βGclp, βGalp.The four fractions had a certain scavenging effect on hydroxyl radical, superoxide anion radical and DPPH radical. When the concentration is 0.1 mg/mL, scavenging rate of AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4 on superoxide anion is about 2 times higher than the positive control vitamin C.

Key words: Residue of astragalus root, Arabinoxylan, Purification, Structural analysis, Antioxidant activity