高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (5): 934-940.doi: 10.7503/cjcu20170464

• 有机化学 • 上一篇    下一篇

铁皮石斛多糖的初级结构分析

高云霄1, 胡小龙1, 王月荣1, 姜志宏2, 章弘扬1, 张敏3, 胡坪1   

  1. 1. 上海市功能性材料化学重点实验室,华东理工大学化学与分子工程学院, 上海 200237
    2. 澳门科技大学中医药学院, 澳门 999078
    3. 上海市新药设计重点实验室, 华东理工大学药学院, 上海 200237
  • 收稿日期:2017-07-14 出版日期:2018-04-18 发布日期:2018-04-18
  • 作者简介:

    联系人简介: 胡 坪, 女, 博士, 教授, 博士生导师, 主要从事药物分析方面的研究. E-mail: huping@ecust.edu.cn;王月荣, 女, 博士, 讲师, 主要从事中药分析方面的研究. E-mail: wangyuerong@ecust.edu.cn

  • 基金资助:
    澳门科技大学中药质量研究国家重点实验室开放课题(批准号: MUST-SKL-2016-06)资助.

Primary Structural Analysis of Dendrobium officinale Polysaccharide

GAO Yunxiao1, HU Xiaolong1, WANG Yuerong1,*, JIANG Zhihong2, ZHANG Hongyang1, ZHANG Min3, HU Ping1,*   

  1. 1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
    2. School of Chinese Medicine, Macau University of Science and Technology, Macao 999078, China
    3. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • Received:2017-07-14 Online:2018-04-18 Published:2018-04-18
  • Contact: WANG Yuerong,HU Ping

摘要:

从新鲜的铁皮石斛中分离得到一种均一的铁皮石斛多糖(SDOP), 分别采用高效凝胶色谱法(GPC)法和1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化法测定其重均分子量和单糖组成, 并通过红外光谱、 甲基化分析和核磁共振波谱对其初级结构进行分析. 结果表明, SDOP的重均分子量为1.66×106, 含有甘露糖和葡萄糖(摩尔比4.9∶1.0). 通过甲基化和核磁共振波谱分析结果推测, SDOP是一种以1,4-链接为主, 存在少量1,2,4-, 1,3,4-, 1,4,6-分支结构和端基结构的O-乙酰化葡甘露聚糖, O-乙酰基主要连接在糖环的2号和3号位.

关键词: 铁皮石斛, 多糖, 结构分析, 核磁共振

Abstract:

A homogeneous polysaccharide(SDOP) was obtained from the fresh Dendrobium officinale. The relative molecular mass and composition of monosaccharide were then determined by gel permeation chromatography-laser scatter(GPC-LS) and high performance liquid chromatography with pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone(PMP) as derivatization agent. The detailed chemical structure of SDOP was elucidated by means of infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy(NMR). The result shows that SDOP with an Mw of 1.66×106 consists dominantly of mannose and glucose in a molar ratio of 4.9∶1.0. The results of NMR and methylation analysis suggest that SDOP is a 1,4-linked O-acetylated glucomannan, with a small amount of branch and end signal, while acetyl groups are mainly attached to the O-2 or O-3 positions on saccharide ring.

Key words: Dendrobium officinale, Polysaccharide, Structure analysis, Nuclear magnetic resonance

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