高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (1): 7.doi: 10.7503/cjcu20150644

• 研究论文: 无机化学 • 上一篇    下一篇

碳酸镧分离富集DNA的研究

康晓燕1, 贺安琪2, 王泾丹2,3, 郭然2, 翟延君1(), 徐怡庄2(), 吴瑾光2   

  1. 1. 辽宁中医药大学药学院, 大连 116600
    2. 北京大学化学与分子工程学院, 北京 100871
    3. 河北师范大学化学与材料科学学院, 石家庄 050024
  • 收稿日期:2015-08-15 出版日期:2016-01-10 发布日期:2015-12-20
  • 基金资助:
    国家自然科学基金(批准号: 51373003, 30873437)和教育部博士点基金(批准号: 20112133110001)资助

Investigation on the Enrichment of DNA Using Lanthanum Carbonate

KANG Xiaoyan1, HE Anqi2, WANG Jingdan2,3, GUO Ran2, ZHAI Yanjun1,*(), XU Yizhuang2,*(), WU Jinguang2   

  1. 1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
    2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
    3. College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2015-08-15 Online:2016-01-10 Published:2015-12-20
  • Contact: ZHAI Yanjun,XU Yizhuang E-mail:lnzyzyj@sohu.com;xyz@pku.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.51373003, 30873437) and the Research Fund for the Doctoral Program of Ministry of Education, China(No;20112133110001)

摘要:

通过紫外-可见分光光度法测定了碳酸镧吸附前后溶液中的DNA含量, 并计算了DNA的吸附率. 通过X射线光电子能谱(XPS)与X射线衍射(XRD)分析了吸附DNA前后固相的变化. 结果表明, 碳酸镧对DNA有较强的吸附作用, 且该过程对碳酸镧晶型未产生明显影响. 通过对吸附条件的考察, 发现DNA的吸附率随pH值升高而降低. 最后通过磷酸二氢钾作为洗脱液将吸附的DNA回收, 洗脱液中镧离子含量低于0.1×10-6 mol/L, 对DNA的后期使用无污染.

关键词: 脱氧核糖核酸, 碳酸镧, 富集, 脱附

Abstract:

The adsorption behavior of Deoxyribonucleic acid(DNA) on lanthanum carbonate particles was investigated. Both UV and XPS results demonstrated that DNA could be adsorbed on the surface of lanthanum carbonate particles. Moreover, the efficiency of adsorption was maximized when the pH value of the solution was set around 5.5. In addition, DNA could be desorpted when lanthanum carbonate particles with adsorpted DNA was dispersed in aqueous solution of KH2PO4. The ICP results demonstrated that the concentration of La3+ was lower than 0.1 μmol/L in the aqueous solution after desorption process, thereby excluding the possibility of undesirable influence on DNA.

Key words: Deoxyribonucleic acid(DNA), Lanthanum carbonate, Enrichment, Desorption

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