胆酸印迹聚合物的制备及在含水介质中的结合性能

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (8): 1727.

胆酸印迹聚合物的制备及在含水介质中的结合性能

许志锋¹,邝代治¹,文戈²,张复兴¹,王剑秋¹,李俊华¹

1. 衡阳师范学院化学与材料科学系, 功能金属有机材料湖南省普通高等学校重点实验室, 衡阳 421008;
2. 紫光古汉集团衡阳中药有限公司, 衡阳 421003

收稿日期:2010-10-11 修回日期:2010-11-01 出版日期:2011-08-10 发布日期:2011-07-19
通讯作者: 许志锋 E-mail:xuzhifenghw@163.com
基金资助:
湖南省高校“十一五”重点建设学科资助项目和衡阳师范学院科研基金(批准号: 2006B04)资助.

Preparation and Binding Properties in Aqueous Media of Cholic Acid Imprinted Polymers

XU Zhi-Feng^1*, KUANG Dai-Zhi¹, WEN Ge², ZHANG Fu-Xing¹, WANG Jian-Qiu¹, LI Jun-Hua¹

1. Department of Chemistry and Material Science, Key Laboratory of Functional Organometallic Materials of College of Hunan Province, Hengyang Normal University, Hengyang 421008, China;
2. Unisplendour Guhan Group Hengyang Chinese Medicines Corporation Limited, Hengyang 421003, China

Received:2010-10-11 Revised:2010-11-01 Online:2011-08-10 Published:2011-07-19
Contact: XU Zhi-Feng E-mail:xuzhifenghw@163.com
Supported by:
湖南省高校“十一五”重点建设学科资助项目和衡阳师范学院科研基金(批准号: 2006B04)资助.

摘要/Abstract

摘要： 为了在含水介质中进行有效印迹，本研究中以双甲基丙烯酰-β-环糊精（BMA-β-CD）和2-(二乙基胺基)乙基甲基丙烯酸酯(DEAEM)为功能单体制备了胆酸印迹聚合物MIP1，并用平衡结合实验研究了MIP1在含水介质中对模板分子的识别能力。结果表明，MIP1比单独以BMA-β-CD或DEAEM为功能单体制备的印迹聚合物MIP2和MIP3，显示出对模板分子更好的选择性结合能力。MIP1的特异性吸附量ΔCP为38.81μmol/g，印迹因子IF为2.46。研究表明，在含水介质中，利用模板分子与功能单体之间的疏水作用和离子作用是提高印迹聚合物分子识别能力的关键。研究还表明，在识别过程中，疏水作用在驱动分子进入印迹孔穴时起重要作用。

关键词: 分子印迹, 胆酸, β-环糊精, 特异性结合, 疏水作用

Abstract: In order to achieve effective imprinting in aqueous media, a cholic acid imprinted polymer (MIP1) was prepared by the combined use of bismethacryloyl-β-cyclodextrin (BMA-β-CD) and 2-(diethylamino) ethylmethacrylate (DEAEM) as functional monomers. The recognition ability of MIP1 for the template in aqueous media was evaluated by equilibrium binding experiments. The results indicated that the imprinted polymer MIP1 obviously exhibited a higher selectivity for cholic acid than the cholic acid imprinted polymers (MIP2 and MIP3) using only BMA-β-CD or DEAEM as a functional monomer. The specific binding capacity (ΔCP) and imprinting factor (IF) of MIP1 were 38.81 μmol/g and 2.46, respectively. It demonstrated that the combination of hydrophobic effect and electrostatic interaction in molecular imprinting was essential for the improvement of the selective ability of the imprinted polymer in aqueous media. In addition, hydrophobic effect was confirmed to be the important force for driving the template molecules into the cavities created by the imprinting process.

Key words: Molecular imprinting, Cholic acid, β-Cyclodextrin, Binding specificity, Hydrophobic effect

中图分类号:

O657.3

TrendMD:

许志锋邝代治文戈张复兴王剑秋李俊华. 胆酸印迹聚合物的制备及在含水介质中的结合性能. 高等学校化学学报, 2011, 32(8): 1727.

XU Zhi-Feng*, KUANG Dai-Zhi, WEN Ge, ZHANG Fu-Xing, WANG Jian-Qiu, LI Jun-Hua. Preparation and Binding Properties in Aqueous Media of Cholic Acid Imprinted Polymers. Chem. J. Chinese Universities, 2011, 32(8): 1727.

参考文献

[1] Zhang H. Q., Piacham T., Drew M., et al. J. Am. Chem. Soc. [J], 2006, 128: 4178-4179 [2] XU Zhi-Feng(许志锋),LIU-Lan(刘岚),HE Jian-Feng(何建锋), et al.. Chem. J. Chinese Universities (高等学校化学学报)[J],2005, 26(11) : 2031-2036 [3] LI-Yong(李永),YANG Huang-Hao(杨黄浩),ZHUANG Zhi-Xia(庄峙厦), et al.. Chem. J. Chinese Universities (高等学校化学学报)[J], 2005，26（9）: 1634-1636 [4] ZHANG Chao-Hui(张朝晖),ZHANG Hua-Bin(张华斌),HU Yu-Fang(胡宇芳), et al.. Scientia Sinica Chimic(中国科学化学) [J], 2009, 39（6）: 525-531 [5] Xu Z. F., LIU L., DENG Q.Y.. Journal of Pharmaceutical and Biomedical Analysis[J], 2006, 41: 701-706. [6] Cong y., Mosbach K.. Journal of Chromatography A[J], 2000, 888: 63-72 [7] Urraca J. L., Moreno-Bondi M. C., Hall A., et al.. Analytical Chemistry[J], 2007,79: 695–701 [8] Lübke C., Lübke M., Whitcombe M. J., et al.. Macromolecules[J], 2000,33: 5098–5105 [9] Wu L. Q., Li Y. Z.. Analytica Chimica Acta[J], 2003,482: 175-181 [10] Hiroyuki A., Masaya K., Masahiko S., et al.. Supramolecular science[J], 1998,5:417-421 [11] Takayuki H., Masahiko S., Masaya K., et al.. Macromolecules[J], 1999,32: 2265-2269 [12] Hishiya T., Asanuma H., Komiyama M.. J. Am. Chem. Soc.[J], 2002, 124（4）: 570-575. [13] Asanuma H., Akiyama T., Kajiya K., et al.. Analytica Chimica Acta[J], 2001, 435: 25-33 [14] Hishiya T., Akiyama T., Asanuma H., et al.. Journal of Inclusion Phenomena and Macrocyclic Chemistry[J], 2002, 44: 365-367 [15] Xu Z. F., Xu L., Kuang D. Z., et al.. Materials Science and Engineering C[J], 2008, 28: 1516–1521 [16] Piletsky S. A., Andersson H. S., Nicholls I. A.. Macromolecules[J], 1999,32: 633-636. [17] Zhu Q. Z., Haupt K., Knopp D., et al.. Anal Chim Acta [J], 2002, 468: 217-227 [18] SU Zhe-Tong(苏柘僮),YANG-Ming(杨明),WU Pin-Jiang(吴品江), et al.. Chinese Traditional Patent Medicine（中成药）[J], 2009,31(3):380-382