磺胺二甲嘧啶分子印迹二维光子晶体水凝胶传感器的研究

doi:10.7503/cjcu20170571

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (2): 212.doi: 10.7503/cjcu20170571

磺胺二甲嘧啶分子印迹二维光子晶体水凝胶传感器的研究

陈小娟, 刘根起(), 任宸锐, 高敏君, 范晓东

西北工业大学理学院应用化学系, 陕西省高分子科学与技术重点实验室, 西安 710129

收稿日期:2017-08-21 出版日期:2018-02-10 发布日期:2017-12-08
作者简介:联系人简介: 刘根起, 男, 博士, 副教授, 主要从事智能凝胶和光子晶体化学传感器方面的研究. E-mail: liugenqi@nwpu.edu.cn
基金资助:
陕西省自然科学基础研究计划项目(批准号: 2017JM5049)和西北工业大学研究生创意创新种子基金(批准号: Z2017209)资助

Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor^†

CHEN Xiaojuan, LIU Genqi^*(), REN Chenrui, GAO Minjun, FAN Xiaodong

Shaanxi Key Laboratory of Macromolecular Science and Technology, Department of Applied Chemistry,School of Science, Northwestern Polytechnical University, Xi’an 710129, China

Received:2017-08-21 Online:2018-02-10 Published:2017-12-08
Contact: LIU Genqi E-mail:liugenqi@nwpu.edu.cn
Supported by:
† Supported by the Natural Science Basic Research Project of Shaanxi Province, China(No.2017JM5049) and the Seed Foundation of Innovation and Creation for Graduate Students in Northwestern Polytechnical University, China(No;Z2017209)

摘要/Abstract

摘要：

以聚苯乙烯二维光子晶体为模板, 磺胺二甲嘧啶为印迹分子, 甲醇为溶剂, 甲基丙烯酸为功能单体, 二甲基丙烯酸乙二醇酯为交联剂, 2,2-二乙氧基苯乙酮为引发剂, 经紫外光引发低温聚合反应, 用甲醇/乙酸(体积比9∶1)混合液洗脱除去印迹分子, 制备得到能够特异性识别磺胺二甲嘧啶的分子印迹二维光子晶体水凝胶(MIPH). 通过测试该水凝胶传感器在不同浓度的磺胺二甲嘧啶溶液中德拜环直径的变化, 考察了其响应性能. 实验结果表明, 当磺胺二甲嘧啶的浓度从0增加到1×10^-6 mol/L时, 德拜环直径增加0.9 cm, 相应的晶格间距减小46 nm. 研究发现, 当MIPH的交联度为10%, 印迹分子与功能单体摩尔比为1∶75时, 德拜环直径变化量最大. 此外, 该水凝胶传感器在浓度为10^-6 mol/L的磺胺二甲嘧啶类似物磺胺嘧啶(SDZ)、磺胺异噁唑(SIZ)及其它抗生素四环素(TE)、罗红霉素(RM)溶液中, 德拜环直径变化量分别为0.4, 0.3, 0.25和0.2 cm, 可见该传感器具有良好的特异性识别功能. 将该传感器用于模拟水样及兽用药片中磺胺二甲嘧啶的测定, 均具有良好的响应性.

关键词: 磺胺二甲嘧啶, 分子印迹, 二维光子晶体, 水凝胶, 传感器

Abstract:

A novel sensor for the rapid and label-free detection of sulfamethazine, based on the combination of photonic crystal and molecular imprinting technique, was developed. The molecularly imprinted photonic hydrogel(MIPH) was prepared using sulfamethazine as the template molecule. The results showed that the diameter of Debye ring increased 0.9 cm when the concentration of SM2 changed from 0 to 1×10^-6 mol/L, namely, the lattice spacing decreased 46 nm. When the ratio of crosslinking of MIPH is 10% and the molar ratio of imprinting molecule to functional monomer is 1∶75, the diameter of Debye ring is the largest. In addition, the diameter of Debye ring only increased 0.2, 0.25, 0.3 and 0.4 cm, respectively when the hydrogel immersed in 1×10^-6 mol/L roxithromycin(RM), tetracycline(TE), sulfonisoxazole(SIZ) and sulfadiazine(SDZ) solution, indicating that the MIPH had a high selectivity. The sensor was also tested for simulated water samples and sulfadimethanol veterinary tablets, and all of them had high responsiveness. The application of such a sensor with high selectivity, high sensitivity, and easy operation might offer a new method for rapid real-time detection of Sulfamethazine.

Key words: Sulfamethazine, Molecularly imprinting, Two-dimensional photonic crystal, Hydrogel, Sensor

中图分类号:

O658

TrendMD:

陈小娟, 刘根起, 任宸锐, 高敏君, 范晓东. 磺胺二甲嘧啶分子印迹二维光子晶体水凝胶传感器的研究. 高等学校化学学报, 2018, 39(2): 212.

CHEN Xiaojuan, LIU Genqi, REN Chenrui, GAO Minjun, FAN Xiaodong. Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor^†. Chem. J. Chinese Universities, 2018, 39(2): 212.

图/表 9

Scheme 1 Schematic illustration for the preparation of MIPH

Fig.1 Illustration of Debye diffraction ring measurement(A) and photograph of Debye ring resulting from diffraction of green laser light by MIPH(B)

Fig.2 SEM images of close packed PS 2D arrays(A) and MIPH(B) The insets are the photographs of PS 2D arrays(A) and MIPH(B)

Fig.3 Dependence of the Debye rings diameter of MIPH(a) and NIPH(b) on the SM2 concentration

Fig.4 Dependence of the particle spacing of the MIPH(a) and NIPH(b) on the SM2 concentration

Fig.5 Schematic of MIPH response to SM2

Fig.6 Change of the Debye rings diameter of MIPH vs. the SM2 concentration prepared with different amounts of crosslinking agenta. 10%; b. 15%; c. 5%; d. 20%.

Fig.7 Repeatibility of MIPH response to 1×10-4 mol/L SM2 solutions and then recovered in a pure phosphate buffer after elution

Fig.8 Selectively recognize of MIPH with different concentrations of SM2(a), SDZ(b), SIZ(c), TE(d) and RM(e)

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磺胺二甲嘧啶分子印迹二维光子晶体水凝胶传感器的研究

Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor^†

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磺胺二甲嘧啶分子印迹二维光子晶体水凝胶传感器的研究

Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor†

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Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor^†