高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (6): 1150-1157.doi: 10.7503/cjcu20180772

• 分析化学 • 上一篇    下一篇

新型磁性限进分子印迹复合材料的制备及富集性能

吴姗姗1, 魏缠玲1,2, 赵丽娟1,2, 田央1, 王霞1, 龚波林1()   

  1. 1. 北方民族大学化学与化学工程学院, 银川 750021
    2. 宁夏出入境检验检疫局综合技术中心, 银川 750002
  • 收稿日期:2018-11-19 出版日期:2019-06-10 发布日期:2019-05-11
  • 作者简介:

    联系人简介: 龚波林, 男, 博士, 教授, 主要从事分离材料及应用研究. E-mail: gongbolin@163.com

  • 基金资助:
    国家自然科学基金(批准号: 21565001, 31271868)资助.

Preparation and Enrichment Properties of Novel Magnetic Restricted Access Media-molecularly Imprinted Composites

WU Shanshan1, WEI Chanling1,2, ZHAO Lijuan1,2, TIAN Yang1, WANG Xia1, GONG Bolin1()   

  1. 1. School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China
    2.Ningxia Entry and Exit Inspection and Quarantine Bureau Comprehensive Technology Center, Yinchuan 750002, China
  • Received:2018-11-19 Online:2019-06-10 Published:2019-05-11
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21565001, 31271868).

摘要:

采用浸渍热解法制备磁性凹凸棒土(MATP), 以磺胺二甲氧嘧啶(SDM)为模板分子, 4-乙烯基吡啶(4-VP)为功能单体, 采用反相原子转移自由基聚合(RATRP)法在MATP表面包覆印迹聚合物薄膜, 得到磁性分子印迹聚合物(MATP-MIPs), 最后在MATP-MIPs表面接枝亲水性单体甲基丙烯酸羟乙酯(HEMA), 得到新型磁性限进分子印迹复合材料(MATP-RAM-MIPs). 采用傅里叶变换红外光谱(FTIR)仪、 扫描电子显微镜(SEM)、 热重分析(TGA)仪、 振动样品磁强计(VSM)和X射线衍射(XRD)仪对材料进行了表征. 通过静态水接触角和考马斯亮蓝G-250方法证明材料外表面具有良好的亲水性和排阻蛋白的特性; 采用静态和选择性吸附实验证实MATP-RAM-MIPs对SDM具有良好的吸附选择性和较高的吸附容量. 将该材料直接用于牛血清中磺胺类药物的富集分离, 加标回收率为86.2%~100.6%, 相对标准偏差为2.1%~4.2%, 可见该材料在复杂样品前处理中具有良好的实际应用价值.

关键词: 磁性凹凸棒土, 反相原子转移自由基聚合, 限进分子印迹聚合物, 吸附性能

Abstract:

The magnetic attapulgite(MATP) was preparrd by pyrolysis method. Then, using sulfadimethoxine(SDM) as template molecule, 4-vinylpyridine(4-VP) as functional monomer, the imprinted layer was coated on the surface of modified MATP via reverse atom transfer radical polymerization(RATRP), getting magnetic molecularly imprinted polymers(MATP-MIPs). Finally, novel magnetic restricted access media-molecularly imprinted composites(MATP-RAM-MIPs) was obtained by grafting hydrophilic monomer hydroxyethyl methacrylate(HEMA) onto the surface of MATP-MIPs. The polymer was characterized by means of Fourier transform infrared(FTIR) spectrum, scanning electron microscope(SEM), thermogravimetric analysis(TGA), vibrating sample magnetometer(VSM) and X-ray diffraction(XRD). The hydrophilicity of MATP-RAM-MIPs and the ability of exclusion of proteins were studied by the water contact angle and Coomassie brilliant blue G-250 method. The adsorption characters of MATP-RAM-MIPs on SDM were studied by adsorption isothermal and selectivity in detail, and MATP-RAM-MIPs were used as solid phase extraction packing to separate and enrichment of SAs from bovine samples. The spiked recoveries were 86.2%—100.6%, and the relative standard deviations(RSD) were 2.1%—4.2%, indicating that this material has application value in the pretreatment of complex samples.

Key words: Magnetic attapulgite, Reverse atom transfer radical polymerization, Restricted access mediamolecularly imprinted polymer, Adsorption property

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