高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (2): 402.

• 研究论文 • 上一篇    下一篇

骨髓基质干细胞在等离子体处理PEGDA基凝胶表面黏附和增殖

谭帼馨1,王迎军2,张姝江3,陈荣1,宁成云2,刘旭建2   

  1. 1. 广东工业大学轻工化工学院应用化学系, 广州 510006; 
    2. 华南理工大学材料学院生物材料研究所, 广州 510640;
    3. 广州医学院第一附属医院, 广州 510120
  • 收稿日期:2010-03-29 修回日期:2010-07-28 出版日期:2010-02-10 发布日期:2011-02-23
  • 通讯作者: 谭帼馨 E-mail:tanguoxin@126.com
  • 基金资助:

    国家自然科学基金(批准号: 50872035)资助.

Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma

TAN Guo-Xin1*, WANG Ying-Jun2, ZHANG Shu-Jiang3, CHENG Rong1, NING Cheng-Yun2, LIU Xu-Jian2   

  1. 1. Department of Applied Chemistry, Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangdong 510006, China;
    2. Institute of Biomaterial, College of Material Science and Engineering, South China University of Technology, Guangdong 510640, China;
    3. The first Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China
  • Received:2010-03-29 Revised:2010-07-28 Online:2010-02-10 Published:2011-02-23
  • Contact: Guo-Xin TAN E-mail:tanguoxin@126.com
  • Supported by:

    国家自然科学基金(批准号: 50872035)资助.

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被引次数

14

摘要： 通过低温等离子处理技术对聚乙二醇双丙烯酸酯（PEGDA）/甲基丙烯酸β-羟乙酯（HEMA）共聚物水凝胶生物材料进行表面改性，以骨髓基质干细胞（BMSc）为细胞模型，考察了细胞在等离子表面改性前后的水凝胶材料的黏附和增值行为. 材料的表面性能通过X射线光电子能谱、接触角仪和扫描电镜进行表征. 研究结果表明，表面经氩等离子处理后，材料的亲水性得到较大的改善，表面自由能由45.9 mJ?m-2增加到70.3 mJ?m-2；体外实验证明，BMSc在等离子处理材料表面培养1天后出现明显细胞核，7天细胞融合成片，通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.

关键词: PEGDA/HEMA, 水凝胶, 等离子表面改性, 细胞黏附和增殖

Abstract: Argon plasma treatment was used to improve the biocompatibility of the surface of photo-polymerization Poly (ethylence glycol) diacrylate(PEGDA)-2-hydroxyethyl methacrylate (HEMA) hydrogel. The adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) on the surface of the films was investigated in this paper. Surface structures of the PEGDA-based films before and after treated by Ar plasma were characterized by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. Results confirm that the surface energy of PEGDA/HEMA hydrogels increased from 45.9 to 70.3 mJ?m-2 after plasma treatment. Compared with the untreated sample, BMSCs spread out more filopodia after 3d incubation on treated films, and it entered cell division phase more quickly. BMSCs culture studies showed that cells grew better on the plasma treated surface, which was beneficial to its cell compatibility.

Key words: PEGDA-HEMA, Hydrogels, Plasma treatment, Adhesion and proliferation

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