高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (9): 1627.

• 研究快报 • 上一篇    下一篇

L-半胱氨酸衍生物配体交换手性色谱固定相

王圣庆1,2, 孟庆华1,2, 郭瑛1, 马言顺1, 龙远德1, 黄天宝1   

  1. 1. 中国科学院成都有机化学研究所,成都610041;
    2. 中国科学院研究生院,北京100049
  • 收稿日期:2005-04-06 出版日期:2005-09-10 发布日期:2005-09-10

A L-Cysteine Derivative as Ligand-exchange Chiral Stationary Phase for Liquid Chromatography

WANG Sheng-Qing1,2, MENG Qing-Hua1,2, GUO Ying1, MA Yan-Shun1, LONG Yuan-De1, HUANG Tian-Bao1   

  1. 1. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2005-04-06 Online:2005-09-10 Published:2005-09-10

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被引次数

27

关键词: 配体交换色谱, 手性拆分, L-半胱氨酸, D, L-&alpha, -氨基酸

Abstract: A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.

Key words: Ligand exchang chromatography, Chiral resolution, L-Cysteine, D,L-α-Amino acid

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