高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1043.

• 研究快报 • 上一篇    下一篇

电穿孔法在量子点标记细胞中的应用

杨蕊1, 冀伟2, 闫玉禧1, 史艳宇3, 费强1, 牟颖1,2, 金钦汉1   

  1. 1. 吉林大学化学学院微分析仪器研究所, 吉林省光谱分析仪器工程技术研究中心, 长春130023;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春130023;
    3. 吉林大学基础医学院, 长春130021
  • 收稿日期:2004-12-21 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 金钦汉(1937年出生),男,教授,博士生导师,从事原子光谱研究.E-mail:qhjin@mai.ljlu.edu.cn; 牟　颖(1969年出生),女,博士,教授,从事光谱学及分子酶学研究.E-mail:ymu100@yahoo.com.cn E-mail:qhjin@mai.ljlu.edu.cn; ymu100@yahoo.com.cn
  • 基金资助:

    国家质检总局重大科技攻关项目(批准号:2003IK057)资助.

Tagging Cells with Quantum Dots by Electroporation

YANG Rui1, JI Wei2, YAN Yu-Xi1, SHI Yan-Yu3, FEI Qiang1, MU Ying1,2, JIN Qin-Han1   

  1. 1. Institute for Miniature Instrumentation, College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Instruments, Jinlin University, Changchun 130023, China;
    2. Key Laboratory for Molecular Enzymology Engineering of Ministry of Education, Jilin University, Changchun 130023, China;
    3. College of Basic Medicine, Jilin University, Changchun 130021, China
  • Received:2004-12-21 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

10

关键词: 量子点, 细胞标记, 电穿孔

Abstract: An electroporation method was proposed to make QDs pass through the membranes of K562 leukaemia cells. This method is operated simply and facilitates the tagging experiment greatly. The results show that when the electroporation voltage is 200 V and the lasting time is 1 ms, a high tagging rate is reached. A lot of cells can be tagged at the same time and the livability of the cells is 60%. The whole process can be completed in 20 min. This method brings forward some more extrusive advantages than cell endocytosis and microinjection method, and there should have a very good foreground for further applications.

Key words: Quantum dot, Cell tagging, Electroporation

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