高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (4): 693-697.doi: 10.7503/cjcu20180635

• 分析化学 • 上一篇    下一篇

超小荧光二硫化钨量子点的水热合成及细胞成像应用

沐亚新, 阿丽, 庄欠粉, 王勇(), 倪永年   

  1. 南昌大学化学学院, 南昌 330031
  • 收稿日期:2018-09-12 出版日期:2019-04-03 发布日期:2019-01-09
  • 作者简介:

    联系人简介: 王 勇, 男, 博士, 副教授, 主要从事分析化学和化学计量学方面的研究. E-mail: wangyong@ncu.edu.cn

  • 基金资助:
    国家自然科学基金(批准号: 21864017, 21305061)、 江西省自然科学基金(批准号: 20181BAB213008, 20171BAB203018)、 江西省教育厅科学技术研究项目(批准号: GJJ160006, GJJ160204)、 中国科学院长春应用化学研究所电分析化学国家重点实验室开放基金(批准号: SKLEAC201802)、 南昌大学食品科学与技术国家重点实验室开放基金(批准号: SKLF-KF-201810)、 湖南大学化学生物传感与计量学国家重点实验室项目(批准号: SKLCBC-2018007)和南昌大学研究生创新专项资金项目(批准号: CX2017040)资助.

Hydrothermal Synthesis of Ultrasmall Fluorescent Tungsten Disulfide Quantum Dots and Their Application in Cell imaging

MU Yaxin, A Li, ZHUANG Qianfen, WANG Yong*(), NI Yongnian   

  1. College of Chemistry, Nanchang University, Nanchang 330031, China
  • Received:2018-09-12 Online:2019-04-03 Published:2019-01-09
  • Contact: WANG Yong E-mail:wangyong@ncu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21864017, 21305061), the Natural Science Foundation of Jiangxi Province, China(Nos.20181BAB213008, 20171BAB203018), the Fund of the Department of Education of Jiangxi Province, China(Nos.GJJ160006, GJJ160204), the Open Funds of the State Key Laboratory of Electroanalytical Chemistry of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences(No.SKLEAC-201802), the Open Project Program of State Key Laboratory of Food Science and Technology of Nanchang University, China(No.SKLF-KF-201810), the Project of the State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University, China(No.SKLCBC-2018007) and the Graduate Student Innovation Program of Nanchang University(No.CX2017040).

摘要:

以钨酸钠和半胱氨酸为原料, 采用水热法一步合成了具有超小粒径(约2 nm)的二硫化钨荧光量子点(WS2 QDs). 利用透射电子显微镜(TEM)、 荧光光谱、 X射线光电子能谱(XPS)、 红外光谱(FTIR)和X射线衍射光谱(XRD)对其进行了表征, 并考察了其稳定性和细胞毒性. 结果表明, 制备的WS2 QDs具有水溶性好、 稳定性高和细胞毒性低的优点. 将此WS2 QDs用于人乳腺癌细胞(MCF-7)的成像, 并通过溶酶体荧光探针进行共定位, 发现此WS2 QDs可能借助溶酶体进入细胞内.

关键词: 二硫化钨量子点, 细胞成像, 荧光

Abstract:

Ultrasmall tungsten disulfide quantum dots(WS2 QDs) were prepared by one-step hydrothermal method using sodium tungstate and cysteine as starting materials. The WS2 QDs were characterized by means of transmission electron microscopy(TEM), fluorescence spectroscopy, infrared spectroscopy and X-ray diffraction spectroscopy(XRD), and their stability and cell toxicity were investigated. The results show that the as-prepared WS2 QDs have good water-solubility, high stability, and low cell toxicity. Then, the WS2 QDs were used for MCF-7 human breast cancer cells imaging, and the results of fluorescence co-localization imaging of Lyso-Tracker Red probes indicate that the WS2 QDs enter the cells via lysosomal pathway.

Key words: Tungsten disulfide quantum dots, Cell imaging, Fluorescence

中图分类号: