高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (8): 1579.doi: 10.7503/cjcu20190076

• 研究论文:无机化学 • 上一篇    下一篇

酪氨酸微波水热法制备碳点及其在Fe3+检测和荧光标记方面的应用

黄贺, 李春光, 施展, 冯守华   

  1. 吉林大学无机合成与制备化学国家重点实验室, 长春 130012
  • 收稿日期:2019-01-23 修回日期:2019-07-23 出版日期:2019-08-10 发布日期:2019-07-12
  • 通讯作者: 李春光,女,博士,高级工程师,主要从事功能无机纳米材料的制备及性能研究.E-mail:cglee@jlu.edu.cn;施展,男,博士,教授,博士生导师,主要从事有机-无机杂化材料研究.E-mail:zshi@mail.jlu.edu.cn E-mail:cglee@jlu.edu.cn;zshi@mail.jlu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号:21771077,21771084,21621001)、国家重点研究发展计划项目(批准号:2016YFB0701100)、高等学校学科创新引智计划(批准号:B17020)和吉林大学高层次科技创新团队项目资助.

Microwave-assisted Hydrothermal Synthesis of Carbon Dots Based on Tyrosine and Their Application in Ion Detection and Bioimaging

HUANG He, LI Chunguang, SHI Zhan, FENG Shouhua   

  1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China
  • Received:2019-01-23 Revised:2019-07-23 Online:2019-08-10 Published:2019-07-12
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos. 21771077, 21771084, 21621001), the National Key Research and Development Program of China(No. 2016YFB0701100), the "111" Project of China(No. B17020) and the Program for JLU Science and Tech-nology Innovative Research Team, China.

摘要: 以酪氨酸为原料,乙二胺为钝化剂,采用绿色、简便的微波辅助水热法制备了尺寸均一、分散性好的碳点.通过调节乙二胺的加入量可以在一定程度上对碳点尺寸进行调节.所得碳点可以通过改变激发波长实现蓝光到绿光的转变,且荧光强度随激发光波长的红移也保持了一定的强度.所得碳点对Fe3+具有很好的选择性猝灭,被用于检测Fe3+时检测限为0.82 nmol/L,检出限为2.98 nmol/L.所制备碳点具有良好的生物相容性和低细胞毒性,被用于标记AD293细胞时标记位置为细胞质.

关键词: 碳点, 微波水热法, Fe3+检测, 细胞标记

Abstract: Cabon dots(CDs) were synthesized utilizing tyrosine as carbon source, ethanediamine as passivant through a green microwave-assisted hydrothermal way. The size of CDs can be adjusted by the dose of ethanediamine. The as-prepared CDs have uniform size and good dispersibility, showing blue or green fluorescence. The as-prepared CDs can change their fluorescence(from blue to yellow) by changing the excitation light and the emission light can be kept without large decay. The as-synthesized CDs have good selectivity on the detection of Fe3+ with a limit of detection(LOD) of 0.82 nmol/L, and a detection limit of 2.98 nmol/L. The as-synthesized CDs also have great biocompatibility and low biotoxicity, and can be utilized as a probe to label AD293 cells, marking the cytoplasm of the cells.

Key words: Carbon dot, Microwave-assisted hydrothermal method, Fe3+ detection, Biomarking

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