高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (2): 231.

• 研究论文 • 上一篇    下一篇

应用纳米磁性球电化学检测特定序列DNA

刘爱丽1, 彭图治1, 程琼1,2   

  1. 1. 浙江大学化学系(西溪校区),杭州 310028;
    2. 嘉兴学院化学工程系,嘉兴 314001
  • 收稿日期:2004-04-15 出版日期:2005-02-10 发布日期:2005-02-10
  • 通讯作者: 彭图治(1946年出生),男,教授,博士生导师,主要从事电分析化学和生物分析化学研究.E-mail:tzp@zju.edu.cn E-mail:tzp@zju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29975024,20275034);浙江省重点科技计划项目(批准号:2003C21024)资助

Electrochemical Detection of DNA Sequences Using Nano-magnetic Particles

LIU Ai-Li1, PENG Tu-Zhi1, CHENG Qiong1,2   

  1. 1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China;
    2. Department of Chemical Engineering, Jiaxing College, Jiaxing 314001, China
  • Received:2004-04-15 Online:2005-02-10 Published:2005-02-10

PDF (PC)

被引次数

47

摘要： 采用分散聚合法制备纳米磁性羧基球,利用化学偶联法将末端修饰氨基的寡聚核苷酸固定在纳米磁性球表面,制成新型核酸探针,该探针可特异性结合目标单链寡聚核苷酸.在磁场作用下,将纳米磁珠与本体溶液分离并富集在电极表面,以中性红为嵌合指示剂,用示差脉冲伏安法测定杂交结果.经过条件优化,本法测定DNA的浓度线性范围为1.0×10-6～5.0×10-9mol/L,检出限为8.6×10-10mol/L.

关键词: 纳米磁性球, 特定序列DNA, 富集, 中性红

Abstract: The carboxyl nanoparticles were prepared by dispersing polymerization method. The oligonucleotides were immobilized on the surface of the nano-magnetic particles by EDC and NHS. The novel DNA probe of magnetic particle can concentrate the trace amount of complementary DNA selectively from the sample solution onto the gold film electrode under a magnetic field. Differential pulse voltammetry was used to detect DNA with an electroactive indicator of neutral red which could bind to double stranded DNA. The linear range for the determination of target DNA is from 1.0×10-6 to 5.0×10-9 mol/L, and the detection limit is found to be 8.6×10-10 mol/L.

Key words: Nano-magnetic particles, DNA sequence, Concentration, Neutral red

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