高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 436.

• 研究论文 • 上一篇    下一篇

通用型激光诱导荧光微流控芯片分析仪的研制与性能考察

周小棉, 戴忠鹏, 林炳承   

  1. 中国科学院大连化学物理研究所, 大连 116023
  • 收稿日期:2003-04-30 出版日期:2004-03-24 发布日期:2004-03-24
  • 通讯作者: 林炳承(1944年出生),男,研究员,博士生导师,从事毛细管电泳和芯片实验室研究.E-mail;bclin@dicp.ac.cn E-mail:bclin@dicp.ac.cn
  • 基金资助:

    国家自然科学基金(批准号;20275039);国家自然科学重点项目基金(批准号;20035010);国家自然科学重大项目基金(批准号;20299030);中国科学院仪器研制专项基金资助

Development and Performance of Laser-induced Fluorescence Microfluidic Chip Analyzer

ZHOU Xiao-Mian, DAI Zhong-Peng, LIN Bing-Cheng   

  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 11063, China
  • Received:2003-04-30 Online:2004-03-24 Published:2004-03-24

摘要: 设计和研制了一种通用型激光诱导荧光微流控芯片分析仪.检测部分按共聚焦检测原理设计,采用CCD(电荷耦合器件)监测通道,三维自动调节聚焦,发射波长滤光片可方便地更换以适应多种染料选择,能分别显示进样和分离通道2条电流-时间曲线.考察了该分析仪的检测灵敏度、检测极限和线性范围,显示了分析灵敏度高,检测限低和线性范围宽等特点,在自制注塑型PMMA塑料芯片上实现了φX174Haedi-gesTdNA片段的分离测定和烟叶act基因PCR产物的分析

关键词: 激光诱导荧光仪, 微流控芯片, 通用型芯片分析仪

Abstract: A laser induced fluorescence microfluidic chip system was developed. The detection system was designed according to the confocal principle. Main features of the system include the alternated emission filter in order to select many dyes, and computer controlled focusing Via 3 D micro manipulator and charge coupled device(CCD), and two curves of the current vs. time and two curves of the voltage vs. time for injection channel and separation channel displayed on the computer′s screen. The detection limit reached 6.67×10-13 mol/L for rhodamine 6G. The linear range of detection was 4.02×10-6 mol/L~4.02×10-9mol/L. The high performance of the system was demonstrated by separation and detection of DNA fragments and PCR products on a home made cast molded PMMA microchip.

Key words: Laser induced fluorescence spectrometer, Microfluidic chip, Microfluidic chip analyzer

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