高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (2): 261.

• 研究快报 • 上一篇    下一篇

微流控芯片停流液-液萃取技术的研究

方群, 陈宏, 蔡增轩   

  1. 浙江大学化学系微分析系统研究所, 杭州 310028
  • 收稿日期:2003-09-02 出版日期:2004-02-24 发布日期:2004-02-24
  • 通讯作者: 方 群(1966年出生),男,博士,副教授,从事微流控分析芯片研究.E-mail;fangqun@mail.hz.zj.cn E-mail:fangqun@mail.hz.zj.cn
  • 基金资助:

    国家自然科学基金重大项目(批准号;20299030);国家“八六三”计划项目(批准号;2002AA2Z2042,2002AA404240);教育部科学技术研究重点项目(批准号;01093)资助

Stopped-flow Liquid-liquid Extraction on Microfluidic Chips

FANG Qun, CHEN Hong, CAI Zeng-Xuan   

  1. Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2003-09-02 Online:2004-02-24 Published:2004-02-24

关键词: 微流控芯片, 液-液萃取, 停流技术

Abstract: In this work, two novel approaches were developed for miniaturized liquid-liquid(L-L) extraction on microfluidic chips, based on a stopped-flow extraction technique. In the first approach, trapped droplets extraction mode, organic solvent droplets of a few hundred 10-12 L were trapped within micro recesses fabricated in the channel walls of microfluidic chips, and analytes in aqueous streams flowing over the droplets were transferred into them, affecting a preconcentration. In the second approach, a stable interface between stationary organic phase and continuously flowed aqueous phase was formed by stopping the flow of organic phase. Analytes were transferred from the aqueous phase into the organic phase on the interface. Enrichment factors exceeding 1 000 and 300 were achieved with a preconcentration period of 20 min with sample consumption lower than 10 μL for trapped droplets and stopped-flow microextraction. In situ laser induced fluorescence detection of the concentrated analyte was performed following the preconcentration.

Key words: Microfluidic chips, Liquid-liquid extraction, Stopped flow technique

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