高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (10): 1889.

• 研究论文 • 上一篇    下一篇

MALDI-TOF质谱和电子光谱技术研究人血清铁蛋白释放铁的动力学

颜利, 黄河清, 金宏伟, 陈平, 杨天赐, 王群力   

  1. 厦门大学生命科学学院, 化学生物学福建省重点实验室, 细胞生物学与肿瘤细胞工程教育部重点实验室, 厦门361005
  • 收稿日期:2003-12-01 出版日期:2004-10-24 发布日期:2004-10-24
  • 基金资助:

    厦门市自然科学基金(批准号:3502Z2001263)资助

Kinetics Study of Iron Release of Human Serum Ferritin with MALDI-TOF Mass Spectrometry and Electronic Spectrum Technology

YAN Li, HUANG He-Qing, JIN Hong-Wei, CHEN Ping, YANG Tian-Ci, WANG Qun-Li   

  1. Key Lab of Chemical Biology of Fujian Province, Key Laboratory of Cell Biology and Tumr Cell Engineering of Ministry of Education, School of Life Sciences, Xiamen University, Xiamen 361005, China
  • Received:2003-12-01 Online:2004-10-24 Published:2004-10-24

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被引次数

9

摘要： 采用MALDI-TOF质谱技术研究血清混合蛋白质的组成,用电子光谱技术分析人血清铁蛋白(Serumferritin,SF)释放铁的动力学过程和规律.结果表明,在人血清中,以Na2S2O4为电子供体时,SF释放铁的速率极快,无法采用常规分光光度法进行检测;以抗坏血酸为电子供体时,SF以一级反应动力学方式释放铁,认为人血清中其它蛋白质协助或参与调控铁蛋白进行快速释放铁反应,从而满足人体对铁的需求.

关键词: 血清, 铁蛋白, 释放铁, 动力学, 质谱

Abstract: MALDI-TOF mass spectrometry was used to understand the compound of proteins in human serum and electronic spectrum was used to study the kinetics of iron release of the human serum ferritin. The result shows that if Na2S2O4 was taken as the reducer, it is impossible to examine the iron release in serum with normal spectrum. When Vc was used as the reducer, the kinetic reaction of the iron release from serum ferritin was the first-order. It might be due to the abundance and influence of other proteins in human serum in the course of iron release, and that could fit the need of iron in the body.

Key words: Serum, Ferritin, Iron release, Kinetics, Mass spectrometry

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