高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (8): 1456.

• 论文 • 上一篇    下一篇

甲醇对微过氧化酶-11电化学行为的影响

王小锋1, 黄晓华1, 姜慧君2, 冯玉英2, 邢巍3, 丁晓岚4, 陆天虹1,3   

  1. 1. 南京师范大学化学与环境科学学院, 南京 210097;
    2. 南京医科大学基础医学院, 南京 210029;
    3. 中国科学院长春应用化学研究所, 长春 130022;
    4. 清华大学生物科学与技术系, 北京 100084
  • 收稿日期:2002-09-18 出版日期:2003-08-24 发布日期:2003-08-24
  • 通讯作者: 陆天虹(1943年出生),男,博士,研究员,博士生导师,从事生物电化学、光谱电化学和化学电源研究.E-mail:tianhonglu@263.net E-mail:tianhonglu@263.net
  • 基金资助:

    国家自然科学基金(批准号:29973015,20243002);国家计划委员会稀土专项基金(批准号:GJX01100626);江苏省科技厅高新技术基金(批准号:BG2001045);江苏省教育厅高校自然科学基金(批准号:01KJB150006,01KJB150004,2000LHZ0

Effect of Methanol on Electrochemical Behavior of Microperoxidase

WANG Xiao-Feng1, HUANG Xiao-Hua1, JIANG Hui-Jun2, FENG Yu-Ying2, XING Wei3, DING Xiao-Lan4, LU Tian-Hong1,3   

  1. 1. Department of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China;
    2. College of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China;
    3. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    4. Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China
  • Received:2002-09-18 Online:2003-08-24 Published:2003-08-24

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被引次数

11

摘要： 用电化学和圆二色性(CD)光谱方法比较了不含和含质量分数为20%甲醇的微过氧化物酶-11(MP-11)溶液的电化学和电催化性能及结构的差别.发现加入甲醇使MP-11的电化学反应的可逆性和对H2O2还原的电催化活性增加,这是由于加入甲醇使部分双聚的MP-11分子变成单体分子,分子的α-螺旋和β-转角的结构含量增加,无规卷曲的结构含量降低,MP-11分子中血红素的暴露程度增加而引起的.

关键词: 微过氧化物酶-11, 甲醇, 催化活性, 圆二色谱

Abstract: The electrochemical and electrocatalytic properties as well as the structures of MP-11 in the solution without and with 20% methanol were compared by using the electrochemical and Circular dichroism methods.It was found that in the presence of methanoLIn the solution, the reversibility of the electrochemical reaction and electrocatalytic activity of MP-11 for the reduction of H2O2increase because in the presence of methanoLIn the solution, the exposure extent of heme in the MP-11 molecule increases, the contents of the α-helix and β-turn structures increase and the content of the random coil structure decreases in the MP-11 molecule.

Key words: Microperoxidase-11, Methanol, Electrocatalytic activity, Circular dichroism spectrum

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