高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (2): 216-220.

• 论文 • 上一篇    下一篇

用水溶液中合成的量子点作为生物荧光标记物的研究

林章碧1, 苏星光1, 张皓2, 牟颖3, 孙晔3, 胡海1, 杨柏2, 闫岗林3, 罗贵民3, 金钦汉1   

  1. 1. 吉林大学化学学院分析科学研究所, 长春分析仪器研究和技术开发中心;
    2. 吉林大学超分子结构与材料重点实验室;
    3. 吉林大学分子酶学工程教育部重点实验室, 长春 130023
  • 收稿日期:2002-07-02 出版日期:2003-02-24 发布日期:2003-02-24
  • 通讯作者: 金钦汉(1937年出生),男,教授,博士生导师,从事光谱分析和微波化学研究.E-mail:crdcai@mai.ljlu.edu.cn苏星光(1966年出生),女,博士,副教授,主要从事光谱分析方面的研究. E-mail:crdcai@mai.ljlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20075009)资助

Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

LIN Zhang-Bi1, SU Xing-Guang1, ZHANG Hao2, MU Ying3, SUN Ye3, HU Hai1, YANG Bai2, YAN Gang-Lin3, LUO Gui-Min3, JIN Qin-Han1   

  1. 1. Institute of Analytical Science, College of Chemistry, Changchun R & D Center for Analytical Instruments;
    2. Key Lab for Supramolecular Structure and Materials;
    3. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, China
  • Received:2002-07-02 Online:2003-02-24 Published:2003-02-24

摘要: 以巯基丙酸(HS—CH2CH2COOH)为稳定剂,在水溶液中合成了具有窄而对称(FWHM=40nm)的荧光发射带且尺寸为3nm的CdTe半导体纳米粒子,并用此纳米粒子成功地标记了生物分子胰蛋白酶.与单独的CdTe纳米粒子的溶液相比较,CdTe-胰蛋白酶溶液的吸收光谱在400~600nm范围内较为平坦,其发射光谱蓝移8nm,但发射峰的半峰宽不变.实验证明,CdTe-胰蛋白酶溶液吸收和发射光谱的变化是由CdTe纳米粒子与胰蛋白酶之间的结合反应引起的,而不是由空气中的O2所引起的,加热可促进CdTe纳米粒子与胰蛋白酶之间的结合反应.

关键词: 量子点, 胰蛋白酶, 生物荧光标记

Abstract: CdTe semiconductor nanoparticles were synthesized in aqueous solution by using mercaptopropyl acid(HS-CH2CH2COOH) as the stabilizer. The nanocrystals exhibit a strong, stable and narrow band luminescence(FWHM=40 nm). The average size estimated from TEM graph is ca. 3 nm. The applicability of the resulting mercaptopropyl acid modified CdTe nanoparticles to the labeling of biomoleculestrypsin was studied in this paper. The absorption spectrum of CdTe-trypsin solution was flatter than that of CdTe nanoparticles over the wavelength range of 400 to 600 nm. While the emission spectrum of CdTe-trypsin solution shows a blue-shift in the emission peak, the intrinsic emission band width is unchanged. It was proved that the shift of the emission peak was the result of the conjugation between CdTe nanoparticles and trypsin, but not of the oxidation caused by O2. Furthermore, heating could accelerate the speed of conjugation between CdTe nanoparticles and trypsin.

Key words: Quantum dot, Trypsin, Biological fluorescent labeling

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