高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (6): 1158-1163.doi: 10.7503/cjcu20180127

• 分析化学 • 上一篇    下一篇

金黄色葡萄球菌活细胞合成ZnSe量子点

张亚楠, 杨玲玲, 涂家薇, 崔然, 庞代文()   

  1. 武汉大学生物医学分析化学教育部重点实验室, 化学与分子科学学院, 病毒学国家重点实验室, 高等研究院, 武汉 430072
  • 收稿日期:2018-02-12 出版日期:2018-06-10 发布日期:2018-05-22
  • 基金资助:
    国家自然科学基金(批准号: 21535005)和湖北省自然科学基金(批准号: 2015CFB401)资助.

Live-cell Synthesis of ZnSe Quantum Dots in Staphylococcus aureus

ZHANG Yanan, YANG Lingling, TU Jiawei, CUI Ran, PANG Daiwen*()   

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, State Key Laboratory of Virology,the Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
  • Received:2018-02-12 Online:2018-06-10 Published:2018-05-22
  • Contact: PANG Daiwen E-mail:dwpang@whu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21535005) and the National Natural Science Foundation of Hubei Province, China(No.2015CFB401).

摘要:

利用“时-空耦合策略”在金黄色葡萄球菌内制得ZnSe量子点, 从而将金黄色葡萄球菌转变成为荧光细胞. 采用透射电子显微镜(TEM)、 原子力显微镜(AFM)、 动态光散射(DLS)、 紫外-可见吸收光谱和荧光发射光谱等对从细胞内提取出的ZnSe量子点进行了表征. 结果表明, 提取出的ZnSe量子点粒径均一、 分散性好,平均粒径为(2.96±0.76) nm, 具有良好的荧光性质, 最大荧光发射波长为537 nm.

关键词: ZnSe, 量子点, 金黄色葡萄球菌, 活细胞, 生物合成

Abstract:

Staphylococcus aureus cells were skillfully utilized to produce ZnSe quantum dots and transformed into fluorescing cells through temporally-spatially coupling strategy. The products were characterized by means of transmission electron microscopy(TEM), atomic force microscope(AFM), dynamic light scattering(DLS), inductively coupled plasma emission spectrometry(ICP-AES), UV-Vis absorption spectroscopy and photoluminescence(PL) spectroscopy. ZnSe QDs biosythesized by such a method possess uniform particle size of (2.96±0.76) nm and good dispersibility with fluorescence emission at 537 nm.

Key words: ZnSe, Quantum dots, Staphylococcus aureus, Live-cell, Biosynthesis

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