高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (1): 46.

• 研究简报 • 上一篇    下一篇

基于有序生物膜的电容型层胶粘蛋白新型免疫传感器

江德臣1, 唐佳1, 刘宝红1, 沈先荣3, 张松1, 杨芃原1, 李爱珍2, 黄宜平2, 孔继烈1   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 复旦大学微电子系, 上海 200433;
    3. 上海海军医药研究所, 上海 200433
  • 收稿日期:2002-01-06 出版日期:2003-01-24 发布日期:2003-01-24
  • 通讯作者: 孔继烈(1964年出生),男,博士,教授,博士生导师,主要从事功能材料光电化学和生物传感器的研究.E-mail:jlkon g@fud an.ac.cn E-mail:jlkon g@fud an.ac.cn
  • 基金资助:

    国家自然科学基金(批准号:39970195,29905001和20005002);长春应用化学研究所电化学国家重点实验室、上海市青年科技启明星计划资助;复旦大学三年行动规划“交叉项目资助计划”资助

A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step

JIANG De-Chen1, TANG Jia1, LIU Bao-Hong1, SHEN Xian-Rong3, ZHANG Song1, YANG Peng-Yuan1, LI Ai-Zhen2, HUANG Yi-Ping2, KONG Ji-Lie1   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Department of Microelectronics, Fudan University, Shanghai 200433, China;
    3. Shanghai Naval Medical Research Institute, Shanghai 200433, China
  • Received:2002-01-06 Online:2003-01-24 Published:2003-01-24

PDF (PC)

被引次数

11

关键词: 层胶粘蛋白, 免疫传感器, 自组装, 电位阶跃

Abstract: Laminine(Ln) has been identified as an important marker of liver fibrosis or cirrhosis. The Ln immunosensor was formed with thioglycolic acid to produce a self-assembled monolayer on the gold electrode surface. The monoclonal Ln antibody was orderly immobilized on the modified gold surface covalently using EDC/NHS. An unlabeled antigen combined on the antibody could reduce the capacitance of the electrode, which was evaluated by the potentiostatic method. The measurement was performed in microseconds. Ln antigen could be determined in the range of 2-50 ng/mL. The quick response made the immunosensor a promising clinical screening method.

Key words: Laminine, Immunosensor, Self-assembly, Potentiostatic step

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