高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (8): 1457.

• 研究论文 • 上一篇    下一篇

四环素-Al(Ⅲ)配合物与DNA的相互作用研究

杨周生, 于俊生, 陈洪渊   

  1. 南京大学配位化学国家重点实验室南京大学化学系, 南京 210093
  • 收稿日期:2001-10-16 出版日期:2002-08-24 发布日期:2002-08-24
  • 作者简介:陈洪渊(1937年出生),男,教授,博士生导师,中国科学院院士,主要从事电分析化学和生物电化学研究.E-mail:hychen@nju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29835110)资助

Studies on Interaction of Tetracycline-Al(Ⅲ) Complex with DNA

YANG Zhou-Sheng, YU Jun-Sheng, CHEN Hong-Yuan   

  1. Department of Chemistry, the State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China
  • Received:2001-10-16 Online:2002-08-24 Published:2002-08-24

PDF (PC)

被引次数

102

摘要： 研究了四环素(TC)-Al3+配合物与DNA的相互作用.吸收光谱和荧光光谱研究表明,TC与DNA无明显的相互作用,但在Al3+离子存在下,TC能结合到小牛胸腺DNA上.TC先与Al3+作用,生成TC-Al二元配合物,该配合物再与DNA发生作用,形成(TC-Al)-DNA三元配合物.TC-Al二元配合物与DNA的结合常数为6.5×104L/mol,其配位比n(TC):n(Al):n(DNA)=1:1:1.TC-Al配合物与DNA的键合模式主要是静电相互作用.溶液的pH值对TC-Al二元配合物和(TC-Al)-DNA三元配合物的形成有一定的影响.利用TC-Al配合物与DNA作用使体系荧光强度增强的性质测定DNA,其线性范围为1.0×10-6～6.0×10-5mol/L,检测限为5.0×10-7mol/L.分别测定了天然与热变性酵母样品中DNA的含量,结果令人满意.

关键词: 四环素, 铝离子, 配合物, DNA

Abstract: The interaction of tetracycline(TC) Al(Ⅲ) complex with DNAwas studied. DNAcould bind to calf thymus DNAwith affinity in the presence of aluminum ions, which have been confirmed by absorption and fluorescence spectra. The increase of peak intensity and red shift of tetracycline in the presence of aluminum ions showed that TC Al binary complex was formed. The spectra changes of TC Al binary complex in the presence of DNAindicate that TC Al binary complex can interact with DNAto form ternary complex with the ratio of n(Al): n(TC): n(DNA)=1:1:1. The binding constant of TC Al complex interacting with DNAwas 6.5×104 L/mol. The formation of binary and ternary complexes could be affected by the pHvalue of the solution. DNAcan also be determined by TC Al binary complex interacting with DNAin the range from 1.0×10-6 to 6.0×105 mol/Lwith the detection limit of 5.0×10-7 mol/L.

Key words: Tetracycline, Aluminum ion, Complex, DNA

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