高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (8): 1453.

• 研究论文 •    下一篇

用荧光光谱和紫外-可见差谱研究抗体-卟啉的相互作用

祁超1,2, 李伟国2, 张玉静1, 刘立岩2, 赵大庆2   

  1. 1. 解放军军需大学生物化学教研室, 长春 130062;
    2. 中国科学院长春应用化学研究所稀土化学与物理开放实验室, 长春 130022
  • 收稿日期:2001-10-22 出版日期:2002-08-24 发布日期:2002-08-24
  • 通讯作者: hychen@nju.edu.cn
  • 作者简介:赵大庆(1963年出生),男,研究员,博士生导师,从事生物无机化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29701005);中国科学院基础局重大项目(批准号:KJ951-A1-504-02);国家杰出青年科学基金(批准号:29625203)资助

Studies on the Interaction of Porphyrin with Antibody with Fluorescent Spectrum and UV-Vis Differential Spectra

QI Chao1,2, LI Wei-Guo2, ZHANG Yu-Jing1, LIU Li-Yan2, ZHAO Da-Qing2   

  1. 1. Depa rtment of Biochemistry, Quartermaster Universityof PL A, Changchun 130062, China;
    2. Key Laboratoryof Rare Earth Chemistryand Physics, Changchun Instituteof Applied Chemistry, Chinese Academy of Scienses, Changchun 130022, China
  • Received:2001-10-22 Online:2002-08-24 Published:2002-08-24

PDF (PC)

被引次数

39

摘要： meso-四(α,α,α,α-O-苯乙酰苯)卟啉与其单克隆抗体1F2结合后,产生显著的增色效应,反映了卟啉与抗体之间刚性紧密的结合,在抗体中的抗原结合部位存在芳香族氨基酸.用同步荧光光谱结合荧光猝灭法得到抗体抗原结合部位的芳香族氨基酸主要为色氨酸和酪氨酸,但酪氨酸的残基数要少于色氨酸.卟啉与抗体的结合比为1:1,解离常数(2.084±0.246)×10-10mol/L,可见卟啉与McAb1F2有很高的亲和力.

关键词: 卟啉, 抗体, 紫外-可见差谱, 荧光光谱

Abstract: After meso tetra (α, α, α, α-O-phenylacetyl benzene)porphyrin combined with McAb1F2, there was a significant hyperchromic effect, indicating that the combination of porphyrin and antibody is rigid and compact, aromatic amino acids exist at the combining sites of antigen in antibody. These aromatic amino acids are Trys and Trps, but the numbers of Trp are more than that found for Trys. The stochiometric ratio of porphyrin to 1F2 is 1:1, the disassociation constant was determined as (2.084 ±0.246)×10-10 mol/Lby a method of fluorescence quenching, showing that both have a high affinity.

Key words: Porphyrin, Antibody, UV-Vis differential spectra, Fluorescent spectra

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