高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1210.

• 研究简报 • 上一篇    下一篇

明胶在聚氨酯表面的固定化及其对内皮细胞生长的促进作用

高长有1, 袁峻2, 管建均1, 沈家骢1   

  1. 1. 浙江大学高分子科学与工程学系, 杭州 310027;
    2. 硅材料国家重点实验室, 杭州 310027
  • 收稿日期:2001-03-28 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 高长有(1966年出生),男,教授,主要从事组织工程材料和自组装研究.E-mail:cygao@mai.lhz.zj.cn E-mail:cygao@mai.lhz.zj.cn
  • 基金资助:

    国家自然科学基金(批准号:59803006);国家重点基础研究发展规划项目(批准号:G1999054305)资助

Immobilization of Gelatin onto Polyurethane Membrane Surface and Acceleration of Endothelial Cells Growth

GAO Chang-You1, YUAN Jun2, GUAN Jian-Jun1, SHEN Jia-Cong1   

  1. 1. Department of Polymer Science and Engineering, Hangzhou 310027, China;
    2. State Key Lab of the Silicone Materiales, Zhejiang University, Hangzhou 310027, China
  • Received:2001-03-28 Online:2002-06-24 Published:2002-06-24

关键词: 聚氨酯, 明胶, 偶联, 内皮细胞, 细胞相容性

Abstract: Gelatin was covalently immobilized onto the polyurethane membrane surface grafted previously with poly(methacrylic acid) (PU-g-PMAA) using 1-[(3-dimethylamino)propyl]-3-ethyl carbodiimide as the condensing reagent. ATR-FTIR and ESCAcharacterizations verify the occurrence of the grafting, and prove that the immobilized amount of gelatin is proportional to the number of active sites, i.e. carboxylic groups on PU surface. Endothelial cells culture reveals that the cell attachment and cell growth on PU-g-PMAA-g-Gelatin are both improved obviously, demonstrating that a cell compatible PUis obtained and the immobilizing method is quite effective to introduce cell growth factors onto polymer surface.

Key words: Polyurethane, Gelatin, Coupling, Endothelial cell, Cytocompatibility

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