高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (11): 1664.

• 研究简报 • 上一篇    下一篇

金表面抗原抗体的固定及其荧光法的测定

姜雄平2, 许丹科1, 刘耀清1, 马立人1   

  1. 1. 军事医学科学院放射医学研究所, 北京 100850;
    2. 解放军总后勤部卫生部药品仪器检验所, 北京 100071
  • 收稿日期:2000-01-21 出版日期:2000-11-24 发布日期:2000-11-24
  • 通讯作者: 许丹科(1965年出生),男,博士,副研究员,主要从事生物传感器等研究.
  • 基金资助:

    中国人民解放军“九五”医药卫生科研基金(批准号:98Z070)资助

Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method

JIANG Xiong-Ping2, XU Dan-Ke1, LIU Yao-Qing1, MA Li-Ren1   

  1. 1. Institute of Radiomedicine, Academy of Military Medical Sciences, Beijing 100850, China;
    2. Institute for Drug Control of PLA, Beijing 100071, China
  • Received:2000-01-21 Online:2000-11-24 Published:2000-11-24

PDF (PC)

被引次数

28

关键词: 免疫传感器, 自组装, 荧光

Abstract: A Gold surface was modified with N-acetylcysteine to produce a self-assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1.0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.

Key words: Immunosensor, Self-assembled monolayer, Fluorescence

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