关键词: 操作式条件反射, 海马, 学习, 氨基酸, 分析

Abstract: Changes of intracellular levels of free amino acids(FAA) in hippocampus of freely moving rats were measured using intracerebral microdialysis coupled to Orthophthaladehyde(OPA) pre-column derivatization reversed phase HPLC-fluorescence detection. The analytical method was systematically optimized and the real samples were determined. It was employed to analyze the trace level of free amino acids(FAA) and possible to qualitative and quantitative 18 kinds of amino acids in every 5μL microdialysate. The sensitivity and the linear relation between the amounts and the peak areas were satisfied. The recoveries were 82%-109% . The detection limits reached 0.5-5.7 pmol for each FAA. A significant decrease of 14 FAAafter training of OCRwas founded in comparison to basal condition(before training). In fore finds of neurotransmitter amino acids(NAA), excitatory NAA, glutamic acid and aspartic acid increased 19.12% and 53.40% respectively than before training. Inhibitory NAA, γ-aminobutyric(GABA), increased 138.17% than before training. The results confirm that metabolic increase of FAAin hippocampus can be induced by learning and memory in rats. The intracerebral microdialysis technique thus appears to be a useful tool to monitor changes in physiological activity of learning and memory during ongoing behavior.

Key words: Operant conditioned reflex, Hippocampus, Learning, Amino acid, Analysis