高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (6): 1127-1138.doi: 10.7503/cjcu20190663

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

细胞膜上信号转导蛋白的单分子成像与分析

梁钰昕,赵容,梁馨月,方晓红*()   

  1. 中国科学院化学研究所, 分子纳米结构与纳米技术重点实验室,北京分子科学国家研究中心, 北京 100190
  • 收稿日期:2019-12-13 出版日期:2020-06-10 发布日期:2020-03-23
  • 通讯作者: 方晓红 E-mail:xfang@iccas.ac.cn
  • 基金资助:
    国家自然科学基金(21735006);国家自然科学基金(21890742);国家自然科学基金(21127901)

Single-molecule Imaging and Analysis of Signal Transduction Proteins on Cell Membranes

LIANG Yuxin,ZHAO Rong,LIANG Xinyue,FANG Xiaohong*()   

  1. Institute of Chemistry, Chinese Academy of Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, Beijing 100190, China
  • Received:2019-12-13 Online:2020-06-10 Published:2020-03-23
  • Contact: Xiaohong FANG E-mail:xfang@iccas.ac.cn
  • Supported by:
    † National Natural Science Foundation of China(21735006);National Natural Science Foundation of China(21890742);National Natural Science Foundation of China(21127901)

摘要:

结合本课题组的研究工作, 介绍了单分子荧光成像原理、 荧光标记方法及数据分析方法, 并进一步综述了单分子荧光成像在几种重要的膜蛋白信号转导分子机制和相关药物研究中的进展.

关键词: 单分子荧光成像, 细胞膜受体, 全内反射荧光显微镜, 超分辨成像, 单分子分析

Abstract:

Membrane proteins play an important role in cell signal transduction. Structural and functional abnormalities of membrane proteins can cause a variety of diseases. The development of single-molecule fluorescence imaging technology has enabled researchers to study the location and dynamic behavior of single membrane proteins in living cell systems, and their interactions with other molecules. Single-molecule fluorescence imaging technology has been widely used to reveal the molecular mechanisms of signal transduction under physiological conditions in recent years. Based on the our research work, this paper introduces the imaging principle, fluorescent labeling method and data analysis method of single-molecule fluorescence imaging in the study of cell membrane proteins, and further reviews the research progress of single-molecule fluorescence imaging on mechanistic study of several important signal transduction membrane proteins and related drug development.

Key words: Single molecule fluorescence imaging, Cell membrane receptor, Total internal reflection fluorescence microscope, Super-resolution imaging, Single molecule analysis

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