高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (2): 326.

• 研究简报 • 上一篇    下一篇

微球载体固定化碱性磷酸酶的底物/产物吸附现象

史国利, 马建标, 何炳林   

  1. 南开大学高分子化学研究所, 天津 300071
  • 收稿日期:1995-02-22 出版日期:1996-02-24 发布日期:1996-02-24
  • 通讯作者: 何炳林
  • 作者简介:史国利,男,29岁,博士.
  • 基金资助:

    国家自然科学基金

Adsorption of Immobilized Alkaline Phosphatase Latex to Substrate and Product

SHI Guo-Li, MA Jian-Biao, HE Bing-Lin   

  1. Institute of Polymer Chemistry, Nankai University, Tianjin 300071
  • Received:1995-02-22 Online:1996-02-24 Published:1996-02-24

PDF (PC)

被引次数

1

关键词: 碱性磷酸酶, 固定化, 底物, 产物, 吸附

Abstract: Aminated polystyrene latex beads were used in immobilization of Calf intestinal alkaline phosphatase.High adsorption of the immobilized-enzyme microbeads to substrate and product of enzyme,i.e.4-nitrophenyl phosphate(pNPP) and 4-nitrophenol(pNP) was found.The adsorption phenomenon had effect in determination of immobilized enzyme activity and the Km value of immobilized enzyme.

Key words: Phosphatase alkaline, Immobilization, Substrate, Product, Adsorption

TrendMD: