高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (6): 924.

• 论文 • 上一篇    下一篇

微量热法研究过氧化氢酶反应

梁毅1, 汪存信1, 吴鼎泉1, 宋昭华1, 屈松生1, 邹国林2   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 武汉大学生命科学院, 武汉, 430072
  • 收稿日期:1994-06-15 修回日期:1994-12-03 出版日期:1995-06-24 发布日期:1995-06-24
  • 通讯作者: 梁毅,男,硕士,讲师,现在武汉化工学院精细化工系工作.
  • 作者简介:梁毅,男,硕士,讲师,现在武汉化工学院精细化工系工作.
  • 基金资助:

    国家自然科学基金

Microcalorimetry Applied to the Study of Catalase Reaction

LIANG Yi1, WANG Cun-Xin1, WU Ding-Quan1, SONG Zhao-Hua1, QU Song-Sheng1, ZOU Guo-Lin2   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. College of Life Science, Wuhan University, Wuhan, 430072
  • Received:1994-06-15 Revised:1994-12-03 Online:1995-06-24 Published:1995-06-24

摘要: 利用微量热法和热动力学方程研究了过氢化氢酶反应.该反应遵循Michaelis-Menten动力学,298.15K和pH7.0时,其米氏常数、酶转换数以及摩尔反应焓分别为2.36×10-2mol/L、1.20×104s-1和-83.67kJ·mol-1.过氧化氢酶反应后期对底物是一级反应,其总反应速度常数和一级速度常数分别为ko=6.31×105L·mol-1·s-1和k1=6.31×105/[Eo]s-1.该反应服从Ogura机理,其酶-底物三元复合物的分解速度常数为6.00×103s-1.

关键词: 微量热法, 过氧化氢酶, 米氏常数, 热动力学方程, 反应机理

Abstract: Microcalorimetry and thermokinetic equations were used to study H2O2decomposition by beef liver catalase.The catalase reaction obeyed the Michaelis-Menten kinetics,and at 298.15 K and pH 7.0,the Michaelis constant (Km),the turnover number and the molar enthalpy(ΔrHm)of the reaction were 2.36×10-2mol/L,1.20×104s-1and -83.67 kJ·mol-1,respectively.The later period of catalase reaction was a first order reaction for H2O2,=6.31×105L·mol-1·s-1and k1=6.31×10-5/[Eo]s-1,respectively.The catalase reaction obeyed Ogura mechanism,and the ternary complex decomposition rate constant was 6.00×103s-1.

Key words: Microcalorimetry, Catalase, Michaelis constant, Thermokinetic equation, Reaction mechanism

TrendMD: