高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (3): 364.

• 论文 • 上一篇    下一篇

血浆中极长链脂肪酸的毛细管气相色谱分析

张抒峰1, 常理文2   

  1. 1. 宁夏大学化学系, 银川;
    2. 中国科学院化学研究所, 北京, 100080
  • 收稿日期:1993-03-15 修回日期:1993-07-20 出版日期:1994-03-24 发布日期:1994-03-24
  • 通讯作者: 张抒峰,女,34岁,讲师
  • 作者简介:张抒峰,女,34岁,讲师

Determination of Very Long Chain Fatty Acids in Plasma by Capillary Gas Chromatography

ZHANG Shu-Feng1, CHANG Li-Wen2   

  1. 1. Department of Chemistry, Ningxia University, Yinchuan, 750021;
    2. Institute of Chemistry, Academia Sinica, 100080
  • Received:1993-03-15 Revised:1993-07-20 Online:1994-03-24 Published:1994-03-24

摘要: 建立了血浆中极长链脂肪酸毛细管气相色谱法,并用于测定了10例正常人和4例临床拟诊为肾上腺白质营养不良(ALD)病人血浆中极长链脂肪酸,其中一例肯定了ALD的临床诊断。

关键词: 极长链脂肪酸, 脂, 血浆, 气相色谱, 肾上腺白质营养不良症

Abstract: Capillary gas chromatographic method was employed for determining very long chain fatty acids in the plasma of patients with Adrenoleucodystrophy(ALD)and healthy controls.Very long chain fatty acids in the plasma were extracted and methylated with CH3OH-C6H6-CH3COCl.The very long chain fatty acids were separated on the SE-54 column.The ratio of C24:0to C22:0,C26:0 to C22:0 fatty acids was calculated.Abnormal ratios were observed in patient with ALD.The technique can be used for clinical diagnosis of ALD.

Key words: Very long chain fatty acids, Lipid, Plasma, Gas chromatography, Adrenoleucodystrophy

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