脂质修饰DNA复合结构的可控制备及膜生物学研究

doi:10.7503/cjcu20200067

高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (6): 1151.doi: 10.7503/cjcu20200067

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇下一篇

脂质修饰DNA复合结构的可控制备及膜生物学研究

张露灏^1,²,曹书婷^1,²,刘江波³,左小磊³,王丽华^1,⁴,樊春海^1,^3,^*(),李江^1,^4,^*()

^1. 中国科学院上海应用物理研究所, 中国科学院微观界面物理与探测重点实验室, 上海 201800
^2. 中国科学院大学, 北京 100049
^3. 上海交通大学医学院分子医学研究院, 附属仁济医院, 上海 200240
^4. 中国科学院上海高等研究院, 张江实验室, 同步辐射光源, 上海 201204

收稿日期:2020-02-10 出版日期:2020-06-10 发布日期:2020-03-23
通讯作者: 樊春海,李江 E-mail:fanchunhai@sjtu.edu.cn;lijiang@zjlab.org.cn
基金资助:
国家自然科学基金(21775157);国家自然科学基金(21834007);国家自然科学基金(21675167)

Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology ^†

ZHANG Luhao^1,²,CAO Shuting^1,²,LIU Jiangbo³,ZUO Xiaolei³,WANG Lihua^1,⁴,FAN Chunhai^1,^3,^*(),LI Jiang^1,^4,^*()

^1. CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
^2. University of Chinese Academy of Sciences, Beijing 100049, China
^3. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200240, China
^4. Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China

Received:2020-02-10 Online:2020-06-10 Published:2020-03-23
Contact: Chunhai FAN,Jiang LI E-mail:fanchunhai@sjtu.edu.cn;lijiang@zjlab.org.cn
Supported by:
† National Natural Science Foundation of China(21775157);National Natural Science Foundation of China(21834007);National Natural Science Foundation of China(21675167)

摘要/Abstract

摘要：

综述了脂质-DNA复合结构的设计、可控制备和结构特性; 并重点讨论其在膜生物学中的应用, 包括对活细胞膜的动态分析、膜上纳米孔道的构建、对活细胞的空间排布与相互作用调控以及活体药物递送等; 总结了该领域存在的一些挑战, 并对未来发展进行了展望. 利用这些精确可控的脂质-DNA复合结构, 研究者可以更深入地理解细胞膜在分子尺度上的工作原理, 实现对细胞膜功能的精确调控, 为细胞成像诊断、纳米机器与人工细胞构建等应用提供有力的工具.

关键词: 脂质-DNA复合结构, 膜锚定, 膜生物学

Abstract:

This review briefly summarized recent progress of lipid-DNA complexes, including their design, construction, and unique features. We highlighted their applications on living cell membranes, including dynamic membrane analysis, nanopores, live cell organization and interaction regulation, and drug delivery. We also summarized challenges in this field. We suggest that by using these lipid-DNA conjugates, cell membrane functions at the molecular level can be better understood and manipulated, which may facilitate the development of cell imaging, theranostics, construction of nanodevices and protocells.

Key words: Lipid-DNA complex, Membrane anchoring, Membrane biology

中图分类号:

Q599

TrendMD:

张露灏, 曹书婷, 刘江波, 左小磊, 王丽华, 樊春海, 李江. 脂质修饰DNA复合结构的可控制备及膜生物学研究. 高等学校化学学报, 2020, 41(6): 1151.

ZHANG Luhao, CAO Shuting, LIU Jiangbo, ZUO Xiaolei, WANG Lihua, FAN Chunhai, LI Jiang. Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology ^†. Chem. J. Chinese Universities, 2020, 41(6): 1151.

图/表 5

Fig.1 Nanopores constructed by lipid-DNA conjugates (A) Self-assembled cholesterol-modified DNA nanopores[37], copyright 2019, American Chemical Society; (B) hydrophobic belts used to construct nanopores[26], copyright 2013, American Chemical Society; (C) porphyrin-modified DNA nanopores[40], copyright 2013, Wiley-VCH; (D) three-cholesterol-modified DNA nanopores[41], copyright 2018, Springer Nature; (E) DNA nanopores constructed from DNA tile structures[42], copyright 2015, American Chemical Society.

Fig.2 Lipid-DNA conjugates regulate cell aggregation (A) Control of cell attachment through lipid-DNA conjugates[60], copyright 2010, Elsevier; (B) lipid modified DNA aptamer mediated cell targeting[63], copyright 2013, Wiley-VCH; (C) programming cellular Interactions by three cholesterol modified DNA nanoplatform[64], copyright 2019, American Chemical Society.

Fig.3 Lipid-DNA conjugates for cell membrane analysis (A) Probing signaling molecules in the cellular membrane microenvironment[66], copyright 2019, Wiley-VCH; (B) phosphorylated lipid-DNA selectively anchors on cell membranes with high alkaline phosphatase expression[67], copyright 2019, Springer Nature.

Fig.4 Lipid-DNA conjugates for studying mechanobiology (A) Lipid modified DNA origami nanoparticles to scaffold and deform lipid membrane [76], copyright 2015, Wiley-VCH; (B) lipid modified DNA-based membrane molecular probes for intercellular tensile forces visualization[95], copyright 2017, American Chemical Society.

Fig.5 Lipid-DNA conjugates for cargo deliveries (A) Lipid modified DNA for in vivo cell modification and localized immunotherapy[103], copyright 2011, Wiley-VCH; (B) membrane encapsulation of DNA nanostructures to achieve in vivo stability[37], copyright 2019, American Chemical Society.

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脂质修饰DNA复合结构的可控制备及膜生物学研究

Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology ^†

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脂质修饰DNA复合结构的可控制备及膜生物学研究

Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology †

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Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology ^†