高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (6): 1151-1162.doi: 10.7503/cjcu20200067

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

脂质修饰DNA复合结构的可控制备及膜生物学研究

张露灏1,2,曹书婷1,2,刘江波3,左小磊3,王丽华1,4,樊春海1,3,*(),李江1,4,*()   

  1. 1. 中国科学院上海应用物理研究所, 中国科学院微观界面物理与探测重点实验室, 上海 201800
    2. 中国科学院大学, 北京 100049
    3. 上海交通大学医学院分子医学研究院, 附属仁济医院, 上海 200240
    4. 中国科学院上海高等研究院, 张江实验室, 同步辐射光源, 上海 201204
  • 收稿日期:2020-02-10 出版日期:2020-06-10 发布日期:2020-03-23
  • 通讯作者: 樊春海,李江 E-mail:fanchunhai@sjtu.edu.cn;lijiang@zjlab.org.cn
  • 基金资助:
    国家自然科学基金(21775157);国家自然科学基金(21834007);国家自然科学基金(21675167)

Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology

ZHANG Luhao1,2,CAO Shuting1,2,LIU Jiangbo3,ZUO Xiaolei3,WANG Lihua1,4,FAN Chunhai1,3,*(),LI Jiang1,4,*()   

  1. 1. CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200240, China
    4. Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China
  • Received:2020-02-10 Online:2020-06-10 Published:2020-03-23
  • Contact: Chunhai FAN,Jiang LI E-mail:fanchunhai@sjtu.edu.cn;lijiang@zjlab.org.cn
  • Supported by:
    † National Natural Science Foundation of China(21775157);National Natural Science Foundation of China(21834007);National Natural Science Foundation of China(21675167)

摘要:

综述了脂质-DNA复合结构的设计、 可控制备和结构特性; 并重点讨论其在膜生物学中的应用, 包括对活细胞膜的动态分析、 膜上纳米孔道的构建、 对活细胞的空间排布与相互作用调控以及活体药物递送等; 总结了该领域存在的一些挑战, 并对未来发展进行了展望. 利用这些精确可控的脂质-DNA复合结构, 研究者可以更深入地理解细胞膜在分子尺度上的工作原理, 实现对细胞膜功能的精确调控, 为细胞成像诊断、 纳米机器与人工细胞构建等应用提供有力的工具.

关键词: 脂质-DNA复合结构, 膜锚定, 膜生物学

Abstract:

This review briefly summarized recent progress of lipid-DNA complexes, including their design, construction, and unique features. We highlighted their applications on living cell membranes, including dynamic membrane analysis, nanopores, live cell organization and interaction regulation, and drug delivery. We also summarized challenges in this field. We suggest that by using these lipid-DNA conjugates, cell membrane functions at the molecular level can be better understood and manipulated, which may facilitate the development of cell imaging, theranostics, construction of nanodevices and protocells.

Key words: Lipid-DNA complex, Membrane anchoring, Membrane biology

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