高等学校化学学报

高等学校化学学报 ›› 1990, Vol. 11 ›› Issue (1): 81.

• 研究简报 • 上一篇    下一篇

酪氨酸为底物的过氧化物酶催化荧光反应

慈云祥, 陈列, 魏姗   

  1. 北京大学化学系
  • 收稿日期:1988-02-23 出版日期:1990-01-24 发布日期:1990-01-24
  • 通讯作者: 慈云祥
  • 基金资助:

    国家自然科学基金

Peroxidase Catalytic Fluorescence Reaction with Tyrosine as Substrate

Ci Yunxiang, Chen Lie, Wei Shan   

  1. Department of Chemistry, Peking University, Beijing
  • Received:1988-02-23 Online:1990-01-24 Published:1990-01-24

PDF (PC)

被引次数

27

摘要： G.G.Guilbault等人曾对过氧化物酶催化H2O2氧化高香草酸(HVA)的反应进行过较详尽的研究[1]。利用这个反应可以对有关的氧化还原酶[2]、辅酶、有机底物、无机离子和微量H2O2进行分析检测。但是,以高香草酸为底物,价格昂贵,作为通用检测方法不够经济。Guilbault认为酪氨酸有类似的反应,且价格便宜,但未进行详细研究。

关键词: 酪氨酸, 辣根过氧化物酶, 荧光

Abstract: Fluorescence behaviours of L-Tyr-H2O2-HRPsystem were investigated. The results indicate that tyrosine is not equal to homovanillic acid for the structure of the substrate, but tyrosine and ho-movanillic acid have the same linear ranges and sensitivities in the enzymatic analysis, and tyrosine can replace homovanillic acid. Therefore, it is possible to make good use of that reaction. The method has been used to determine glucose and tyrosine in human sera and the results are obtained satisfactory.

Key words: Tyrosine, Horse radish peroxidase, Fluorescence

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