高等学校化学学报

高等学校化学学报

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Dawson型多金属氧酸盐-维生素C复合物对α-葡萄糖苷酶的抑制作用及机制研究

黄馨怡,阙茂梅,李瑶,雷珊,林思琪,王力   

  1. 集美大学 海洋食品与生物工程学院
  • 收稿日期:2025-08-28 修回日期:2025-11-23 网络首发:2025-11-26 发布日期:2025-11-26
  • 通讯作者: 王力 E-mail:wanglimerry@jmu.edu.cn
  • 基金资助:
    国家自然科学基金项目(批准号：22271119)资助

Inhibition Mechanism of α-Glucosidase by Dawson-Type Polyoxometalate-Vitamin C Complexes

HUANG Xinyi, QUE Maomei, LI Yao, LEI Shan, LIN Siqi, WANG Li   

  1. College of Marine Food and Biological Engineering, Jimei University
  • Received:2025-08-28 Revised:2025-11-23 Online First:2025-11-26 Published:2025-11-26
  • Supported by:
    Supported by the National Natural Science Foundation of China (No.22271119)

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摘要： 本研究对Dawson型多金属氧酸盐和维生素C进行复合，以探究复合物对α-葡萄糖苷酶的抑制作用.合成并表征了两类(母体和铁、钴、镍3种过渡金属取代的磷钼酸共4个)Dawson型磷钼酸H6[P2Mo18O62]，H8[P2Mo17Fe(OH2)O61]，H8[P2Mo17Co(OH2)O61]和H8[P2Mo17Ni(OH2)O61](以下简称为P2Mo18，P2Mo17Fe，P2Mo17Co和P2Mo17Ni)，在探究以上4个Dawson型磷钼酸对α-葡萄糖苷酶抑制作用的基础上，通过Dawson型磷钼酸与维生素C以不同比例复合，进行相应的酶动力学实验，考察不同比例的4个Dawson型磷钼酸与维生素C复合物对α-葡萄糖苷酶的抑制效果，并选取最优抑制效果比例进行相对应的抑制机理和抑制类型的研究. 结果表明4个不同的Dawson型磷钼酸-VC复合物均对α-葡萄糖苷酶有良好的抑制作用. P2Mo18-VC复合比例为5:1时、P2Mo17Fe-VC和P2Mo17Ni-VC复合比例为3:1时、P2Mo17Co-VC复合比例为2:1时对α-葡萄糖苷酶抑制效果最好,4种Dawson型磷钼酸-VC复合物（P2Mo18，P2Mo17M-VC(M=Ni2+，Co2+，Fe2+)均为可逆的混合型抑制，结果表明4个多酸与VC的复合物具有开发为α-葡萄糖苷酶抑制剂的潜力.

关键词: 多金属氧酸盐, α-葡萄糖苷酶; 维生素C；酶动力学；酶抑制剂

Abstract: This study investigated the inhibitory effects of Dawson-type polyoxometalates (POMs) complexed with vitamin C (VC) on α-glucosidase activity. Four Dawson-type phosphomolybdates were synthesized and characterized, including the parent compound H?[P?Mo??O??] (P?Mo??) and three transition metal-substituted derivatives (H?[P?Mo??Fe(OH?)O??] (P?Mo??Fe), H?[P?Mo??Co(OH?)O??] (P?Mo??Co), and H?[P?Mo??Ni(OH?)O??] (P?Mo??Ni)). Enzyme kinetic studies revealed that all four POM-VC complexes exhibited significant inhibitory activity against α-glucosidase. The optimal inhibition ratios were determined to be 5:1 for P?Mo??-VC, 3:1 for P?Mo??Fe-VC and P?Mo??Ni-VC, and 2:1 for P?Mo??Co-VC, with corresponding IC?? values of 0.194 mM, 2.507 mM, 2.809 mM, and 5.332 mM, respectively. Mechanistic studies demonstrated that these complexes functioned as reversible mixed-type inhibitors. These findings suggest the potential of Dawson-type POM-VC complexes as novel α-glucosidase inhibitors for diabetes management.

Key words: Polyoxometalates, α-Glucosidase, Vitamin C, Enzyme inhibition, Kinetic

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