高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (11): 2426-2434.doi: 10.7503/cjcu20209236

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

大斯托克斯位移远红光至近红外荧光探针用于检测肝损伤过程中过氧化亚硝酸盐的动态变化

吴倩,程丹,吕芸,袁林,张晓兵   

  1. 湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2020-07-18 出版日期:2020-11-10 发布日期:2020-11-06
  • 基金资助:
    国家自然科学基金(批准号(21877029);21735001, 21904035)和中国博士后科学基金(2018M642968)

Monitoring of Peroxynitrite Variation During Liver Injury Adopting a Far Red to Near-infrared Fluorescent Probe with Large Stokes Shift

WU Qian, CHENG Dan, LÜ Yun, YUAN Lin(), ZHANG Xiaobing   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China
  • Received:2020-07-18 Online:2020-11-10 Published:2020-11-06
  • Contact: YUAN Lin E-mail:lyuan@hnu.edu.cn
  • Supported by:
    This paper is supported by the National Natural Science Foundation of China(21877029);the China Postdoctoral Science Foundation(2018M642968)

摘要:

肝损伤是影响公众健康的重大问题之一, 已经引起了人们越来越多的关注. 而过表达的过氧化亚硝酸盐(ONOO?)在肝损伤等疾病的发病机制中起着重要作用, 被认为是一种与早期肝损伤密切相关的生物活性分子. 因此, 为了探究ONOO?在肝损伤过程中的作用, 开发可以实现肝损伤过程中ONOO?高选择性和实时检测的分析方法具有重要意义. 本文报道了一种具有大斯托克斯位移的远红光至近红外(FR-NIR)ONOO?荧光探针. 由于该探针具有大的斯托克斯位移, 可以有效消除光谱重叠和自吸收的干扰, 从而显著提高成像的信噪比. 此外, 该探针对ONOO?具有高的灵敏度(检出限为25.8 nmol/L)和良好的选择性, 被成功用于药物诱导肝损伤过程中ONOO?信号的成像检测.

关键词: 荧光探针, 大斯托克斯位移, 过氧化亚硝酸盐, 药物诱导肝损伤

Abstract:

Liver injury is one of the major problems affecting public health, and has attracted growing attention. It is universally believed that overexpression of peroxynitrite(ONOO?) plays an important role in the pathogenesis of liver injury and other diseases, and it is closely related to early liver injury. Therefore, in order to understand the role of ONOO? in the process of liver injury, an analysis method that can achieve high selectivity and real-time detection of ONOO? is urgently developed. Herein, we present a far red to near-infrared(FR-NIR) fluorescent probe with large Stokes shift to detect the ONOO? production in vivo during drug induced liver injury(DILI). Owing to the large Stokes shift, the FR-NIR probe(LSDQ-ONOO?) can not only reduce the interference of spectral overlap and self-absorption, but also improve the imaging signal-to-noise ratio. High sensitivity(detection limit=25.8 nmol/L) and excellent selectivity ensured that LSDQ-ONOO? can monitor subtle changes of ONOO?in vitro and in vivo during DILI.

Key words: Fluorescent probe, Large Stokes shift, Peroxynitrite, Drug induced liver injury

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