高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (3): 442-448.doi: 10.7503/cjcu20140813

• 分析化学 • 上一篇    下一篇

北五味子对糖尿病脑病大鼠脑中神经活性物质含量影响的在线微透析-高效液相色谱-串联质谱联用分析

皮子凤1, 王倩倩1, 张静2, 宋凤瑞1, 刘志强1()   

  1. 1. 中国科学院长春应用化学研究所国家质谱中心(长春), 吉林省中药化学与质谱重点实验室, 长春 130022
    2. 吉林大学药学院, 长春 130021
  • 收稿日期:2014-09-09 出版日期:2015-03-10 发布日期:2015-01-30
  • 作者简介:联系人简介: 刘志强, 男, 博士, 研究员, 博士生导师, 主要从事天然药物化学与有机质谱学研究. E-mail: liuzq@ciac.ac.cn
  • 基金资助:
    国家自然科学基金(批准号: 81373952)和国家科技部创新方法工作专项(批准号: 2012IM030600)资助

Effect of Schisandra Fruit on Neurochemicals in Hippocampus of Diabetic Encephalopathy Rat Using Online MD-HPLC-MS/MS

PI Zifeng1, WANG Qianqian1, ZHANG Jing2, Song Fengrui1, LIU Zhiqiang1,*()   

  1. 1. National Center of Mass Spectrometry, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022, China
    2. School of Pharmaceutical Science, Jilin University, Changchun 130021, China
  • Received:2014-09-09 Online:2015-03-10 Published:2015-01-30
  • Contact: LIU Zhiqiang E-mail:liuzq@ciac.ac.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.81373952) and the Innovation Method Fund of Ministry of Science and Technology, China(No.2012IM030600)

摘要:

采用在线微透析-高效液相色谱-串联质谱联用方法, 测定了糖尿病脑病大鼠大脑海马区的8种脑递质的含量, 从脑中神经活性物质的角度研究五味子改善学习记忆能力的作用机制. 实验结果表明, 经五味子治疗后, 痴呆大鼠脑透析液中的谷氨酸(Glu)、 丝氨酸(Ser)、 多巴胺(DA)及5-羟色胺(5-HT)的含量显著降低(P<0.05), 牛磺酸(Tau)及乙酰胆碱(Ach)的含量显著升高(P<0.01), 天冬氨酸(Asp)和γ-氨基丁酸(GABA)的含量有降低趋势(P>0.05), 8种神经活性物质的水平均向正常水平发生了调节. 此结果说明五味子可能通过调节糖尿病大鼠大脑中神经活性物质的含量发挥保护中枢神经系统的作用, 从而改善糖尿病脑病大鼠的学习记忆能力. Morris水迷宫实验结果表明, 五味子水提物可以明显缩短糖尿病脑病大鼠的逃避潜伏期, 增加穿越目标区域次数及中心区域(%)(P<0.05).

关键词: 五味子, 在线微透析-高效液相色谱-串联质谱联用, 糖尿病脑病大鼠, 海马区, 神经活性物质

Abstract:

The results of morris water maze test showed that the extract of schisandra fruit could significantly shorten the escape latency time(ELT) and increase the time of crossing target zone and central zone(%) of diabetic encephalopathy rats. In order to investigate the mechanism of schisandra fruit on the improving ability of learning and memory, online MD-HPLC-MS/MS method was applied to explore the effect of schisandra fruit on eight neurochemicals changes in hippocampus of diabetic encephalopathy rats. The results showed that the contents of Glu, Ser, DA and 5-HT decreased significantly(P<0.05), while the contents of Tau and Ach increased significantly(P<0.01) after treated by schisandra fruit. The contents of Asp and GABA tended to decrease but the difference was not significant(P>0.05). The levels of eight neurochemicals regulated to normal level. The results indicated that schisandra may play a protective function of the central nervous system by adjusting the contents of neurochemicals in diabetic rats, which can improve the memory and learn ability of diabetic encephalopathy rat.

Key words: Schisandra fruit, Online MD-HPLC-MS/MS, Diabetic encephalopathy rat, Hippocampus, Neurochemical

中图分类号: