高等学校化学学报 ›› 2022, Vol. 43 ›› Issue (2): 20210595.doi: 10.7503/cjcu20210595

• 分析化学 • 上一篇    下一篇

部分还原氧化石墨烯的制备、 表征及对人宫颈癌HeLa细胞的体外杀伤作用

王雪丽1, 宋相伟2, 解颜宁1, 杜妮阳1, 王振新3()   

  1. 1.吉林工商学院粮食学院, 长春 130507
    2.长春师范大学生命科学学院, 长春 130032
    3.中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2021-08-18 出版日期:2022-02-10 发布日期:2021-10-27
  • 通讯作者: 王振新 E-mail:wangzx@ciac.ac.cn
  • 基金资助:
    国家自然科学基金(21475126);吉林省科技发展计划项目(20190304051YY);吉林省教育厅项目(JJKH20210879KJ);吉林工商学院项目(批准号: KB2019[001])和长春师范大学自然科学基金(批准号: 长师大自然合字[2018]第12号)资助

Preparation, Characterization of Partially Reduced Graphene Oxide and Its Killing Effect on Human Cervical Cancer Cells

WANG Xueli1, SONG Xiangwei2, XIE Yanning1, DU Niyang1, WANG Zhenxin3()   

  1. 1.Grain College, Jilin Business and Technology College, Changchun 130507, China
    2.Life Science of College, Changchun Normal University, Changchun 130032, China
    3.Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022, China
  • Received:2021-08-18 Online:2022-02-10 Published:2021-10-27
  • Contact: WANG Zhenxin E-mail:wangzx@ciac.ac.cn
  • Supported by:
    the National Natural Science Foundation of China(21475126);the Science and Technology Development Program of Jilin Province, China(20190304051yy);the Project of the Education Department of Jilin Province, China(JJKH20210879KJ);the Project of Jilin Business and Technology College, China(No.KB2019[001]) and the Natural Science Foundation of Changchun Normal University, China (No.Chang Shi Da He Zi [2018] No.12)

摘要:

以氧化石墨烯(GO)为原料, 利用温和方法制备了3种不同还原程度的部分还原氧化石墨烯pRGO1, pRGO2和pRGO3(pRGO1—3); 利用傅里叶变换红外光谱(FTIR)、 拉曼光谱(Raman)、 X 射线光电子能谱(XPS)、 紫外-可见光谱(UV-Vis)、 透射电子显微镜(TEM)和 EDS能谱对其结构和形貌进行了表征. 细胞实验结果表明, 无激光照射下pRGO1—3本身的细胞毒性较低; 近红外(NIR)激光照射下pRGO1—3通过光热和光毒性双重作用杀伤肿瘤细胞. 实验结果显示了pRGO 在肿瘤光热疗法和光动力疗法领域的应用潜力.

关键词: 部分还原氧化石墨烯, 宫颈癌细胞, 光热治疗, 光毒性治疗

Abstract:

Partially reduced grphene oxides(pRGO1—pRGO3, pRGO1—3) were synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room tempeature. Structure and morphology of the as-synthesized pRGO1—3 nanocomposites were confirmed by means of FTIR, Raman, XPS, UV-Vis, transmission electron microscopy(TEM) and energy dispersive spectrometer(EDS).pRGO possesses synergistic photodynamic and photothermal effects on tumor cell upon near-infrared(NIR) light irradiation. Consequently, pRGO can be expected to be a new nano carrier for tumor photodynamic and photothermal therapy.

Key words: Partially reduced graphene oxide, Human cervical cancer cells, Photothermal therapy, Phototoxicity therapy

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