高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (3): 439-447.doi: 10.7503/cjcu20180563

• 分析化学 • 上一篇    下一篇

基于金属-有机骨架Fe-MIL-88NH2的凝血酶适体传感器

李德蕾, 谷梦巧, 王敏, 迟宽能, 张茜, 邓燕, 马玉婵, 胡蓉(), 杨云慧()   

  1. 云南师范大学化学化工学院, 昆明 650500
  • 收稿日期:2019-08-10 出版日期:2019-01-24 发布日期:2019-01-24
  • 作者简介:

    联系人简介: 胡 蓉, 女, 博士, 副教授, 主要从事电分析化学与生物传感器方面的研究. E-mail: hudierong_168@163.com; 杨云慧, 女, 博士, 教授, 主要从事电分析化学与生物传感器方面的研究. E-mail: yyhui2002@aliyun.com

  • 基金资助:
    国家自然科学基金(批准号: 21765026, 21465026)资助.

Preparation of Thrombin Aptasensor Based on the Metal-organic Framework Fe-MIL-88NH2

LI Delei, GU Mengqiao, WANG Min, CHI Kuanneng, ZHANG Xi, DENG Yan, MA Yuchan, HU Rong*(), YANG Yunhui*()   

  1. College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China
  • Received:2019-08-10 Online:2019-01-24 Published:2019-01-24
  • Contact: HU Rong,YANG Yunhui E-mail:hudierong_168@163.com;yyhui2002@aliyun.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21765026, 21465026).

摘要:

合成了一种含氨基的金属-有机骨架材料Fe-MIL-88NH2, 并研制一种基于Fe-MIL-88NH2的适用于快速检测凝血酶的三明治型适体传感器. 以掺杂金纳米颗粒的共价有机框架材料Au NPs@COF-LZU-8复合材料作为固定基质固定凝血酶适体Ⅰ, 凝血酶与适体Ⅰ特异性结合后, 再与通过戊二醛交联制得的凝血酶适体Ⅱ(Apt)-Fe-MIL-88NH2复合物结合, 采用差分脉冲伏安法直接检测标记物Fe-MIL-88NH2中铁离子(Fe3+)的还原峰电流. Fe-MIL-88NH2中Fe3+的电流响应值与凝血酶浓度在0.5~200 ng/mL范围内呈正比, 线性相关系数为0.987, 检测限为0.167 ng/mL. 该传感器简化了实验步骤, 扩大了MOFs的应用范围, 为设计生物传感器开辟了新思路.

关键词: 凝血酶, 金属有机骨架, Fe-MIL-88NH2, 适体传感器, 三明治型

Abstract:

A sandwich-type aptasensor based on the metal-organic framework Fe-MIL-88NH2 for the rapid detection of thrombin was developed. Amino-functionalized metal-organic framework(Fe-MOFs), Fe-MIL-88NH2, was used as a label, which was covalently cross linked with amino-modified thrombin aptamer Ⅱ through glutaraldehyde. Gold nanoparticle modified covalent organic frameworks(Au NPs@COF-LZU-8) with high electronic conductivity was employed as the substrate to immobilize thiolated thrombin aptamer Ⅰ(TBAⅠ). After thrombin binds specifically with TBAⅠ, it combines with Fe-MIL-88NH2 labeled thrombin aptamer Ⅱ. Then, iron ions(Fe3+) from Fe-MIL-88NH2 were directly detected without acid dissolution and preconcentration. Under the optimized experimental conditions, the current response of Fe3+ in Fe-MIL-88NH2 was directly proportional to the concentration of thrombin in the range of 0.5—200 ng/mL, with a linear correlation coefficient of 0.987 and a detection limit of 0.167 ng/mL. In this strategy, the detecting steps were greatly simplified. This article expands the application range of MOFs.

Key words: Thrombin, Fe-metal-organic framework, Fe-MIL-88NH2, Aptasensor, Sandwich type

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