聚乙二醇化学改性的大豆分离蛋白凝胶

doi:10.7503/cjcu20170449

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (2): 390.doi: 10.7503/cjcu20170449

聚乙二醇化学改性的大豆分离蛋白凝胶

刘杰¹, 周浩²(), 黄郁芳³, 陈新¹()

1. 聚合物分子工程国家重点实验室, 复旦大学高分子科学系, 先进材料实验室, 上海 200433
2. 复旦大学附属眼耳鼻喉科医院, 上海 200031
3. 复旦大学材料科学系, 上海 200433

收稿日期:2017-07-11 出版日期:2018-02-10 发布日期:2018-01-08
作者简介:联系人简介: 陈新, 男, 博士, 教授, 博士生导师, 主要从事生物大分子材料的研究. E-mail: chenx@fudan.edu.cn; 周浩, 男, 副教授, 副主任医师, 主要从事眼科临床及生物医用材料的研究. E-mail: zheent@139.com
基金资助:
国家自然科学基金(批准号: 21274028, 21574023, 21574024)资助

Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel^†

LIU Jie¹, ZHOU Hao^2,^*(), HUANG Yufang³, CHEN Xin^1,^*()

1. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science,Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
2. Eye and ENT Hospital, Fudan University, Shanghai 200031, China
3. Department of Materials Science, Fudan University, Shanghai 200433, China

Received:2017-07-11 Online:2018-02-10 Published:2018-01-08
Contact: ZHOU Hao,CHEN Xin E-mail:zheent@139.com;chenx@fudan.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.21274028, 21574023, 21574024)

摘要/Abstract

摘要：

将聚乙二醇单甲醚中的羟基氧化成醛基, 然后通过Schiff 碱及还原反应将聚乙二醇片段接枝到大豆分离蛋白的分子链上. 经过聚乙二醇改性的大豆分离蛋白可以在37 ℃自发形成凝胶, 且随着聚乙二醇接枝率的增加, 凝胶时间可以缩短至30 min以内. 因此, 经过该乙二醇改性的大豆蛋白水凝胶有望成为一种可注射型水凝胶, 在生物医药领域具有相当的应用前景.

关键词: 大豆分离蛋白, 聚乙二醇单甲醚, 化学改性, 水凝胶

Abstract:

Soy protein isolate(SPI), a kind of plant protein, has attracted comprehensive interests in many fields due to its sustainability, low cost, functionality, biocompatibility and tunable degradability. However, SPI needs to take an appropriate modification to meet the requirement of practical use because of the weak intermolecular interactions among the polypeptide chains due to its globular protein nature. Therefore, we designed a chemical modification method to change the aggregation state of SPI molecular chains and prepared a SPI-based hydrogel thereafter. Firstly, polyethylene glycol monomethyl ether(mPEG-CHO) was synthesized by Tempo-BAIB method, achieving an average oxidation degree of 56%. Then, mPEG was successfully grafted onto SPI through Schiff’s base reaction between mPEG-CHO and the amino groups in SPI molecular chains, followed by a reduction reaction from NaCNBH₄. It was found that such a PEG modified SPI solution(10%) was able to form a hydrogel at 37 ℃ spontaneously. In the meantime, it showed that with the increase in grafting ratio, the gelation time can be within 30 min. Such a characteristic make this SPI-based hydrogel an injectable hydrogel, which may have the considerable potential in the biomedicine field. However, the mechanical properties of current PEG modified SPI hydrogel is still not high enough(storage modulus of 300 Pa), so further effort should be taken to make it meet the requirement of real applications.

Key words: Soy protein isolate, Polyethylene glycol monomethyl ether, Chemical modification, Hydrogel

中图分类号:

O636

TrendMD:

刘杰, 周浩, 黄郁芳, 陈新. 聚乙二醇化学改性的大豆分离蛋白凝胶. 高等学校化学学报, 2018, 39(2): 390.

LIU Jie, ZHOU Hao, HUANG Yufang, CHEN Xin. Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel^†. Chem. J. Chinese Universities, 2018, 39(2): 390.

图/表 7

Scheme 1 Synthesis of mPEG-CHO

Fig.1 FTIR(A) and 1H NMR(B) spectra of mPEG-OH(a) and mPEG-CHO(b)

Scheme 2 Synthesis of mPEG modified SPI

Fig.2 FTIR(A) and 1H NMR spectra(B) of pristine SPI(a), SPI-mPEG10∶1(b) and SPI-mPEG20∶1(c)

Fig.3 TEM images of pristine SPI(A) and mPEG modified SPI(B)

Fig.4 Optical images of pristine SPI(A, B) and mPEG modified SPI(C, D) solution before(A, C) and after(B, D) incubation at 37 ℃ Solution concentration: 10%.

Fig.5 Rheological test of mPEG modified SPI hydrogel at 37 ℃ (A) Strain sweep; (B) angular frequency sweep.

参考文献 24

[1]	Kinsella J. E., J. Am. Oil Chem. Soc., 1979, 56, 242—258
[2]	Song F., Tang D. L., Wang X. L., Wang Y. Z., Biomacromolecules,2011, 12(10), 3369—3380
[3]	Zhao S., Yao J. R., Fei X., Shao Z. Z., Chen X., Mater. Lett., 2013, 95, 142—144
[4]	Koshy R. R., Mary S. K., Thomas S., Pothan L. A., Food Hydrocolloid., 2015, 50, 174—192
[5]	Barac M. B,Stanojevic S.P.,Jovanovic S. T.,Pesic M. B., Acta Periodica Technologica, 2004, 35, 3—16
[6]	Kharkar P. M., Kiick K. L., Kloxin A. M., Chem. Soc. Rev., 2013, 42(17), 7335—7372
[7]	Yoshizawa K., Mizuta R., Taguchi T., Biomaterials,2015, 63, 14—23
[8]	Harvey D., Bardelang P., Goodacre S. L., Cockayne A., Thomas N. R., Adv. Mater., 2017, 29, 4245—4250
[9]	Diao C., Xia H., Parnas R. S., ACS Appl. Mater. Interfaces, 2015, 7(40), 22601—22609
[10]	Gokarn Y. R., McLean M., Laue T. M., Mol. Pharm., 2012, 9(4), 762—773
[11]	Hao S. J., Wang Y. J., Kang A. J., Liu Y. D., Li X. N., Shi H., Ma R. Y., Ma G. H., Su Z. G., Chem. J. Chinese Universities, 2010, 31(11), 2239—2245
	(郝素娟, 汪音爵, 康爱君, 刘永东, 李秀男, 石红, 马润宇, 马光辉, 苏志国. 高等学校化学学报, 2010,31(11), 2239—2245)
[12]	Shingel K. I., Di Stabile L., Marty J. P., Faure M. P., Int. Wound J., 2006, 3(4), 332—342
[13]	Ma L., Yang Y. H., Yao J. R., Shao Z. Z., Chen X., Polym. Chem., 2013, 4(21), 5425—5431
[14]	Masson C., Scherman D., Bessodes M., J. Polym. Sci. Part A: Polym. Chem., 2001, 39(22), 4022—4024
[15]	Freitas D. D., Mero A., Pasut G., Bioconj. Chem., 2013, 24(3), 456—463
[16]	Dal Pozzo A., Vanini L., Fagnoni M., Guerrini M., De Benedittis A., Muzzarelli R., Carbohydr. Polym., 2000, 42(2), 201—206
[17]	Shi X., Tan L., Xing J., Cao F., Chen L., Luo Z., Wang Y., J. Appl. Polym. Sci., 2013, 128(3), 1995—2002
[18]	Bendrea A. D., Fabregat G., Cianga L., Estrany F., del Valle L. J., Cianga I., Alemán C., Polym. Chem., 2013, 4(9), 2709—2723
[19]	Dong X. M., Yuan B. M., Chen B. P., He C. L., Wang J. C., Chen X. S., Chem. J. Chinese Universities, 2017, 38(5), 866—871
	(董晓明, 袁宝明, 陈炳鹏, 贺超良, 王金成, 陈学思. 高等学校化学学报, 2017,38(5), 866—871)
[20]	Tian K., Shao Z. Z., Chen X., Biomacromolecules,2010, 11(12), 3638—3643
[21]	Alconcel S. N., Baas A. S., Maynard H. D., Polym. Chem., 2011, 2(7), 1442—1448
[22]	Ma L., Yang Y., Yao J. R., Shao Z. Z., Huang Y., Chen X., J. Mater. Chem. B, 2015, 3(26), 5241—5248
[23]	Nishinari K., Fang Y., Guo S., Phillips G. O., Food Hydrocolloid., 2014, 39, 301—318
[24]	Ren K., He J. L., Zhang M. Z., Wu Y. X., Ni P. H., Acta Chim. Sincia, 2015, 73(10), 1038—1046
	(任锴, 何金林, 张明祖, 吴一弦, 倪沛红. 化学学报, 2015,73(10), 1038—1046)

聚乙二醇化学改性的大豆分离蛋白凝胶

Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel^†

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聚乙二醇化学改性的大豆分离蛋白凝胶

Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel†

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Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel^†