高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (7): 1269-1275.doi: 10.7503/cjcu20160116

• 分析化学 • 上一篇    下一篇

基于铜离子与DNA相互作用的铜离子检测

李敏, 孔慧芳, 郭志慧()   

  1. 陕西师范大学化学化工学院, 陕西省生命分析化学重点实验室, 西安710119
  • 收稿日期:2016-02-28 出版日期:2016-07-10 发布日期:2016-06-15
  • 基金资助:
    基国家自然科学基金(批准号: 21575085)资助

Detection of Copper Ion Based on the Interaction Between DNA Molecules and Copper Ions

LI Min, KONG Huifang, GUO Zhihui*()   

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi’an 710119, China
  • Received:2016-02-28 Online:2016-07-10 Published:2016-06-15
  • Contact: GUO Zhihui E-mail:zhguo@snnu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21575085)

摘要:

利用铜离子(Cu2+)可与DNA分子中的碱基相互作用形成络合物的性质, 将Cu2+富集在DNA修饰电极表面, 进而采用微分脉冲伏安法(DPV)实现了铜离子的检测. 此外, 由于乙二胺四乙酸(EDTA)对Cu2+具有更强的络合能力, 富集于DNA修饰电极表面的Cu2+很容易被洗脱液中的EDTA络合, 从而实现修饰电极的再生和重复利用. 实验结果表明, 在最佳实验条件下, Cu2+ 浓度在2.0×10-6~1.0×10-5 mol/L和2.0×10-5~1.0×10-4 mol/L范围内与其相对还原峰电流强度(I-I0)呈良好的线性关系, 且该传感器简单、 稳定, 可循环使用.

关键词: DNA修饰电极, 电化学传感器, 差分脉冲伏安法, 铜离子

Abstract:

Copper ion(Cu2+) is one of the essential trace elements of human, which plays an important role in life. However, excessive amounts of copper ions may cause serious damage to health. In this work, Cu2+ ions were determined by differential pulse voltammetry(DPV) based on its concentration on DNA modified electrode due to the complexation of Cu2+ ions with the base of DNA. Furthermore, because ethylenediaminetetraacetic acid(EDTA) shows stronger complexing ability to Cu2+ ions than DNA, Cu2+ concentrated on DNA modified electrode can be eluted with EDTA. Thus, the modified electrode was reusable. The results showed that under optimum conditions, the relative reduction current(I-I0) was linear with the concentration of copper ions in the range of 2.0×10-6—1.0×10-5 mol/L and 2.0×10-5—1.0×10-4 mol/L. The proposed electrochemical sensor for Cu2+ ions was simple, stable and reusable.

Key words: DNA modified electrode, Electrochemical sensor, Differential pulse voltammetry, Copper ion

中图分类号: