高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (5): 860.doi: 10.7503/cjcu20150938

• 分析化学 • 上一篇    下一篇

盐酸阿霉素分子印迹传感器的制备及识别特性

张燕, 郑晶, 王娟, 郭满栋()   

  1. 山西师范大学化学与材料科学学院, 临汾 041004
  • 收稿日期:2015-12-17 出版日期:2016-05-10 发布日期:2016-04-12
  • 作者简介:联系人简介: 郭满栋, 男, 教授, 主要从事生物传感器与电分析化学研究. E-mail:guomd@dns.sxnu.edu.cn
  • 基金资助:
    山西省自然科学基金(批准号: 20001057)资助

Preparation and Properties of Doxorubicin Hydrochloride Sensor Based on Molecularly Imprinted Polymer

ZHANG Yan, ZHENG Jing, WANG Juan, GUO Mandong*()   

  1. College of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, China
  • Received:2015-12-17 Online:2016-05-10 Published:2016-04-12
  • Contact: GUO Mandong E-mail:guomd@dns.sxnu.edu.cn
  • Supported by:
    † Supported by the Natural Science Foundation of Shanxi Province, China(No.20001057)

摘要:

采用自组装以及电聚合的方法, 在磷酸盐缓冲液(PBS)中以3,4-乙烯二氧噻吩(EDOT)为功能单体, 盐酸阿霉素(DOX)为模板, 在金电极表面电聚合制备DOX印迹敏感膜(MIPs), 构建了一种选择性检测DOX的分子印迹电化学传感器. 采用循环伏安法(CV)及交流阻抗法(EIS)对其性能进行了表征. 优化实验条件后, 在含0.005 mol/L K3 [Fe(CN)6]及0.1 mol/L KCl的PBS中, 应用差分脉冲伏安法(DPV)测试了该传感器的响应性能. 实验结果表明, 该传感器检测DOX的线性范围为4.0×10-7~1.0×10-6 mol/L, 相关系数为0.9967, 检出限(S/N=3)达6.5×10-8 mol/L; 采用电化学洗脱法可使传感器再生, 对DOX的测定具有良好重现性及稳定性; 该传感器对于干扰物长春碱、 放线菌素D及5-氟尿嘧啶有微弱的电流响应, 显示出良好的选择性. 将该传感器用于人体血样中盐酸阿霉素的分析, 回收率为96.0%~106.7%, 表明其具有潜在的实用价值.

关键词: 分子印迹聚合物, 电化学传感器, 3, 4-乙烯二氧噻吩, 盐酸阿霉素

Abstract:

An electrochemical sensor was developed for the determination of doxorubicin hydrochloride(DOX) using the molecularly imprinted technique. A molecular imprinted polymer(MIP) on the surface of a gold electrode was prepared by electropolymerization of 3,4-ethylenedioxythiop-hene(EDOT) in the presence of DOX in the phosphate buffer solution. Under the optimum conditions, the properties of the electrochemical sensor modified with DOX-imprinted membrane were characterized by cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. A linear relationship between oxidation peak current and DOX concentration was obtained over the range of 4.0×10-7—1.0×10-6 mol/L with a correlation coefficient of 0.9967 and a detection limit(S/N=3) of 6.5×10-8 mol/L. After regeneration by washing with electrochemical method, the sensor showed excellent reproducibility and good stability. The MIP electrode exhibited very weak response to vinblastine, actinomycin D and fluorouracile, proving a good selectivity. The imprinted sensor was applied to the determination of doxorubicin hydrochloride in human blood serum samples with the relative standard deviation(RSD) below 4% and recovery ranging from 96.0% to 106.7%.

Key words: Molecularly imprinted polymer, Electrochemical sensor, 3, 4-Ethylene dioxythiophene, Doxorubicin hydrochloride

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