高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (5): 902-906.doi: 10.7503/cjcu20160626

• 高分子化学 • 上一篇    

高效抗菌Cu2+-壳聚糖季铵盐/丙烯酸复合物的制备

李会, 邢哲, 李荣, 张明星, 王明磊, 谭奕哲, 吴国忠   

  1. 中国科学院上海应用物理研究所, 上海 201800
  • 收稿日期:2016-09-05 修回日期:2017-04-21 出版日期:2017-05-10 发布日期:2017-04-20
  • 通讯作者: 吴国忠,男,博士,研究员,博士生导师,主要从事高分子材料辐射改性、壳聚糖的辐射降解及产业化应用及海水提铀研究.E-mail:wuguozhong@sinap.ac.cn E-mail:wuguozhong@sinap.ac.cn
  • 基金资助:

    上海市科技兴农重点攻关项目(批准号:G20140308)资助.

Preparation of Cu2+-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity

LI Hui, XING Zhe, LI Rong, ZHANG Mingxing, WANG Minglei, TAN Yizhe, WU Guozhong   

  1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  • Received:2016-09-05 Revised:2017-04-21 Online:2017-05-10 Published:2017-04-20
  • Supported by:

    Supported by the Shanghai Agriculture Applied Technology Development Program, China(No.G20140308).

摘要:

以过硫酸铵为引发剂,在壳聚糖季铵盐(HACC)上接枝丙烯酸(AAc)并络合Cu2+,制备了具有高效抗菌性能的HACC-g-PAAc-Cu2+复合物.采用红外光谱(FTIR)和核磁共振波谱(1H NMR)表征了壳聚糖季铵盐接枝改性前后的化学结构变化;利用紫外-可见吸收光谱(UV-Vis)、Cu2+选择电极和热失重分析(TGA)表征了壳聚糖季铵盐接枝前后负载Cu2+的能力;测定了HACC-Cu2+和HACC-g-PAAc-Cu2+对金黄色葡萄球菌及大肠杆菌的最小抑菌浓度,并对小鼠经口摄入毒性和兔皮肤敷涂刺激性进行了考察.研究结果表明,在壳聚糖季铵盐上负载Cu2+能够有效提高其抗菌性;接枝丙烯酸能提高HACC负载Cu2+的能力和抗菌性,Cu/HACC结构单元的摩尔比由接枝前的3:7提高到接枝后的1:1;对金黄色葡萄球菌的最小抑菌浓度由60 mg/L下降到9.2 mg/L,对大肠杆菌的最小抑菌浓度由37 mg/L下降到6.3 mg/L,无摄入毒性和皮肤刺激性.

关键词: 壳聚糖季铵盐, 铜离子, 抗菌性, 丙烯酸

Abstract:

Acrylic acid(AAc) was grafted to chitosan quaternary ammonium salt(HACC) by ammonium persulfate as an initiator. HACC-g-PAAc-Cu2+ with efficient antibacterial activity was further prepared through complexation of Cu2+ with HACC-g-PAAc. The chemical structure of the grafted HACC was characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance(1H NMR). The capacity of grafted HACC loading Cu2+ was determined by ultraviolet-visible spectroscopy(UV-Vis) and thermagravimetric analysis(TGA). Antimicrobial property of HACC-Cu2+ and HACC-g-PAAc-Cu2+ against staphylococcus aurous(S. Aurous) and E. coli bacillus were tested. Toxicity for oral intake of mice and coating irritation on rabbit skin of HACC-Cu2+ and HACC-g-PAAc-Cu2+ were also evaluated. The property of antibacterial and capacity of complexing Cu2+ can be improved by grafting of AAc. The molar ratio of Cu2+/HACC increased from 3:7 before grafting to 1:1 after grafting. The minimum inhibitory concentration(MIC) of S. Aurous and E. coli bacillus decreased from 60 mg/L to 9.2 mg/L and 37 mg/L to 6.3 mg/L. Moreover, HACC-Cu2+ and HACC-g-PAAc-Cu2+ have no toxic ingestion and skin irritation.

Key words: Chitosan quaternary ammonium salt, Cupric ion, Antibacterial property, Acrylic acid

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