高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (1): 43.doi: 10.7503/cjcu20150513

• 有机化学 • 上一篇    下一篇

脱除阿魏酸后车前子多糖的溶液性质、 构象特征及固体形貌

殷军艺, 刘晓莹, 聂少平, 谢明勇()   

  1. 南昌大学食品科学与技术国家重点实验室, 南昌 330047
  • 收稿日期:2015-07-01 出版日期:2016-01-10 发布日期:2015-12-20
  • 基金资助:
    国家自然科学基金(批准号: 31301434, 21564007)、 “十二五”国家科技支撑计划项目(批准号: 2012BAD33B06)、 教育部高等学校博士学科点专项科研基金(批准号: 20133601120009)和江西省自然科学基金(批准号: 20142BAB214004)资助

Solution, Conformational Properties and Morphology of Psyllium Polysaccharide with Ferulic Acid Removal

YIN Junyi, LIU Xiaoying, NIE Shaoping, XIE Mingyong*()   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2015-07-01 Online:2016-01-10 Published:2015-12-20
  • Contact: XIE Mingyong E-mail:myxie@ncu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.31301434, 21564007), the National Key Technology Research and Development Program of China for the 12th Five-year Plan(No.2012BAD33B06), the Research Fund for the Doctoral Program of Higher Education of China(No.20133601120009) and the Natural Science Foundation of Jiangxi Province, China(No.20142BAB214004)

摘要:

分别采用NaOH和阿魏酸酯酶对车前子多糖(PLCP)进行脱除阿魏酸处理, 得到的多糖分别记为PLCP-FAS和PLCP-FAE. 通过基本理化性质测定和单糖组成分析比较了处理前后多糖的基本结构特征, 并采用乌式黏度计、 高效体积排阻色谱(HPSEC)和扫描电子显微镜(SEM)等方法表征了多糖的溶液性质、 构象特征及固体形貌. 结果表明, 经NaOH或阿魏酸酯酶处理后多糖的单糖组成基本不变, 但糖含量和糖醛酸含量增加, 表观黏度显著下降; PLCP-FAS和PLCP-FAE在0.1 mol/L NaCl和0.5 mol/L NaOH中的特性黏度基本相同; HPSEC分析结果表明, PLCP-FAE的重均分子量(Mw)为3.17×106, PLCP-FAS的Mw为2.83×106, PLCP-FAS和PLCP-FAE的持续围长值一致, 但相同分子量下PLCP-FAS的特性黏度低于PLCP-FAE; SEM测定结果显示, PLCP-FAS和PLCP-FAE仍保留一定线性链特征. 上述结果表明, 脱除阿魏酸的大粒车前子多糖呈现半柔顺链的构象特征, 样品固体形貌仍保留一定的线性链特征.

关键词: 车前子, 多糖, 阿魏酸, 溶液性质, 构象特征

Abstract:

This study was aimed at investing the solution and conformation properties of polysaccharide from psyllium(or seeds of Plantago asiatica L.). The fraction which was subjected with NaOH to remove ferulic acid was named as PLCP-FAS, while another fraction was PLCP-FAE whose ferulic acid was removed by feruloyl esterase. Ubbelohde capillary viscometer, HPSEC with refractive index, light scattering and viscometric detectors and scanning electron microscope(SEM) were applied to investigate the two fractions’ solution, conformation properties and solid form. The results showed that there was little change in monosaccharide compositions of the polysaccharide after ferulic acid removing, not mater by NaOH or enzyme. Sugar contents and uronic acid contents were much higher both for the two fractions. However, its apparent viscosity reduced remarkably. Intrinsic viscosity of PLCP-FAS and PLCP-FAE were close either in 0.1 mol/L NaCl or 0.5 mol/L NaOH. HPSEC analysis results showed that weight average molecular weight of PLCP-FAS was 3.17×106, while it was 2.83×106 for PLCP-FAE. Persistence length of PLCP-FAS and PLCP-FAE were similar. SEM results showed that both PLCP-FAE and PLCP-FAS still exhibited certain linear chain characteristics. The above results indicated that the psylliun polysaccharide after removing ferulic acid was semi flexible, and morphology of the polysaccharide remained some linear chain characteristics.

Key words: Psyllium, Polysaccharide, Ferulic acid, Solution property, Conformation characteristic

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