高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (1): 37-42.doi: 10.7503/cjcu20150434

• 分析化学 • 上一篇    下一篇

L-色氨酸分子印迹光子晶体水凝胶膜的制备及性能

杨兆昆, 张晓栋, 施冬健, 陈明清, 刘士荣()   

  1. 江南大学化学与材料工程学院, 食品胶体与生物技术教育部重点实验室, 无锡 214122
  • 收稿日期:2015-06-01 出版日期:2016-01-10 发布日期:2015-11-17
  • 基金资助:
    国家自然科学基金(批准号: 51173072)资助

Preparation and Characterization of L-Tryptophan Molecularly Imprinted Photonic Hydrogels

YANG Zhaokun, ZHANG Xiaodong, SHI Dongjian, CHEN Mingqing, LIU Shirong*()   

  1. School of Chemical and Material Engineering, The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
  • Received:2015-06-01 Online:2016-01-10 Published:2015-11-17
  • Contact: LIU Shirong E-mail:liushirong1@aliyun.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.51173072)

摘要:

采用胶体晶体模板法, 借助“三明治”结构制备了可与基底剥离的分子印迹光子晶体水凝胶膜(MIPHs). 该MIPHs以L-色氨酸(L-Trp)为模板分子, 丙烯酰胺(AM)为功能单体, N,N'-亚甲基双丙烯酰胺(BIS)为交联剂, 在紫外光下引发聚合. 扫描电子显微镜(SEM)表征结果表明, MIPHs具有相互贯通的三维有序大孔结构. 制得的MIPHs在L-Trp的缓冲溶液中可快速响应, 当L-Trp的浓度从10-10 mol/L增大到10-5 mol/L时, MIPHs的Bragg衍射峰位移83 nm, 并伴有明显的颜色变化. 此外, MIPHs在L-Trp结构类似物L-酪氨酸(L-Tyr)和L-苯丙氨酸(L-Phe)的缓冲溶液中Bragg衍射峰位移较小, 表明制得的MIPHs具有良好的选择性.

关键词: “三明治”结构, 反蛋白石, 光子晶体, 分子印迹, L-色氨酸

Abstract:

Free-standing molecularly imprinted photonic hydrogels(MIPHs) that peeled from the supporters were prepared using the colloidal crystal template method assisted by “sandwich structure”. MIPHs were fabricated by UV polymerization with L-Tryptophan(L-Trp) as template molecule, acrylamide(AM) as functional monomer, N,N'-methylene bisacrylamide(BIS) as crosslinking agent. MIPHs were characterized by highly ordered three-dimensional interconnected macroporous structure. MIPHs showed rapid response property when immersed into L-Trp buffers. The Bragg diffraction peak of MIPHs redshifted 83 nm as the concentration of L-Trp increased from 10-10 mol/L to 10-5 mol/L, and the color changes could be visible by the naked eyes. Moreover, MIPHs showed only a slight shift in the Bragg diffraction peak in solutions of L-Tyr or L-Phe, which indicated that MIPHs possessed a certain selectivity. High selectivety, rapid response and signal self-reporting of MIPHs might provide great convenience for the application in the real-world environments.

Key words: “Sandwich”, structure, Inverse opal, Photonic crystal, Molecularly imprinted, L-Tryptophan

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