L-色氨酸分子印迹光子晶体水凝胶膜的制备及性能

doi:10.7503/cjcu20150434

摘要/Abstract

摘要：

采用胶体晶体模板法, 借助“三明治”结构制备了可与基底剥离的分子印迹光子晶体水凝胶膜(MIPHs). 该MIPHs以L-色氨酸(L-Trp)为模板分子, 丙烯酰胺(AM)为功能单体, N,N'-亚甲基双丙烯酰胺(BIS)为交联剂, 在紫外光下引发聚合. 扫描电子显微镜(SEM)表征结果表明, MIPHs具有相互贯通的三维有序大孔结构. 制得的MIPHs在L-Trp的缓冲溶液中可快速响应, 当L-Trp的浓度从10^-10 mol/L增大到10^-5 mol/L时, MIPHs的Bragg衍射峰位移83 nm, 并伴有明显的颜色变化. 此外, MIPHs在L-Trp结构类似物L-酪氨酸(L-Tyr)和L-苯丙氨酸(L-Phe)的缓冲溶液中Bragg衍射峰位移较小, 表明制得的MIPHs具有良好的选择性.

关键词: “三明治”结构, 反蛋白石, 光子晶体, 分子印迹, L-色氨酸

Abstract:

Free-standing molecularly imprinted photonic hydrogels(MIPHs) that peeled from the supporters were prepared using the colloidal crystal template method assisted by “sandwich structure”. MIPHs were fabricated by UV polymerization with L-Tryptophan(L-Trp) as template molecule, acrylamide(AM) as functional monomer, N,N'-methylene bisacrylamide(BIS) as crosslinking agent. MIPHs were characterized by highly ordered three-dimensional interconnected macroporous structure. MIPHs showed rapid response property when immersed into L-Trp buffers. The Bragg diffraction peak of MIPHs redshifted 83 nm as the concentration of L-Trp increased from 10^-10 mol/L to 10^-5 mol/L, and the color changes could be visible by the naked eyes. Moreover, MIPHs showed only a slight shift in the Bragg diffraction peak in solutions of L-Tyr or L-Phe, which indicated that MIPHs possessed a certain selectivity. High selectivety, rapid response and signal self-reporting of MIPHs might provide great convenience for the application in the real-world environments.

Key words: “Sandwich”, structure, Inverse opal, Photonic crystal, Molecularly imprinted, L-Tryptophan

中图分类号:

O658
O648.1

TrendMD:

杨兆昆, 张晓栋, 施冬健, 陈明清, 刘士荣. L-色氨酸分子印迹光子晶体水凝胶膜的制备及性能. 高等学校化学学报, 2016, 37(1): 37.

YANG Zhaokun, ZHANG Xiaodong, SHI Dongjian, CHEN Mingqing, LIU Shirong. Preparation and Characterization of L-Tryptophan Molecularly Imprinted Photonic Hydrogels^†. Chem. J. Chinese Universities, 2016, 37(1): 37.

图/表 8

Scheme 1 Schematic illustration of the procedure for the preparation of L-MIPHs

Fig.1 SEM images of PS colloidal crystals(A) and inverse opals of MIPHs(B)

Fig.2 Optical response of L-MIPHs(A) and NIPHs(B) upon soaking in L-Trp buffers^c/(mol·L-1): a. 0; b. 10-10; c. 10-9; d. 10-8; e. 10-7; f. 10-6; g.10-5.

Fig.3 Color change induced by the rebinding of L-Trp at different concentrations^ (A) Pure buffer; (B)—(G) concentration of L-Trp/(mol·L-1): 10-10, 10-9, 10-8, 10-7, 10-6, 10-5.

Fig.4 Influence of of imprinting molecule content on the sensing property of L-MIPHs^Content of L-Trp imprinted/(mmol·L-1): a. 0; b. 0.1; c. 0.2; d. 0.3.

Fig.5 Influence of the crosslinking monomer(BIS) content on the sensing property of L-MIPHs^n(BIS)∶n(AM): a. 4%; b. 6%; c. 8%.

Fig.6 Selectivity of L-MIPHs to L-Tyr(A), L-Phe(B), D-Trp(C) and plots of Bragg diffraction peak shifts for L-MIPHs to L-Trp(■), D-Trp(●), L-Tyr(▲), L-Phe(△) and NIPHs to L-Trp(□)(D)^c/(mol·L-1): a. 0; b. 10-10; c. 10-9 ; d. 10-8; e. 10-7; f. 10-6; g. 10-5.

Fig.7 Kinetic response of L-MIPHs when immersed into 10-5 mol/L L-Trp buffers(A) and recoverability of MIPHs incubated in 10-5 mol/L L-Trp buffers and then recovered in deionized water(B)

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