碳纤维微电极负载金纳米粒子用于芦荟多糖的在体动态监测

doi:10.7503/cjcu20190422

摘要/Abstract

摘要：

建立了一种对活体芦荟储水凝胶组织中芦荟多糖进行在体实时动态检测的电化学方法. 通过在活体芦荟植株凝胶中嵌入高灵敏度的纳米金粒子修饰的碳纤维微电极, 对不同光照条件下芦荟植株中的芦荟多糖进行在体动态监测, 进而评价植株应激外界环境下芦荟多糖含量的实时变化. 实验结果表明, 纳米金粒子修饰的碳纤维微电极对芦荟储水凝胶组织中芦荟多糖具有显著的电催化活性, 不同光照环境下生长的芦荟植株中芦荟多糖含量的动态变化具有显著的统计学差异. 该修饰电极可用于芦荟植株中芦荟多糖动态变化的在体实时监测, 使电化学检测方法有望成为监测植物光合作用程度的一种快捷检测手段.

关键词: 碳纤维微电极, 纳米金粒子, 芦荟多糖, 动态监测

Abstract:

An electrochemical method for real-time dynamic detection of aloe polysaccharide in water storage tissue gel layer of aloe leaves was established. The aloe polysaccharides in aloe plants under different growth conditions were monitored in vivo by high-sensitivity carbon fiber microelectrodes modified with gold nanoparticles(AuNPs/CFME), to evaluate the real-time changes of aloe polysaccharides in the environment under stress. The experimental results show that the AuNPs/CFME has significant electrocatalytic activity to aloe polysaccharides in aloe water storage tissue. The dynamic changes of aloe polysaccharide in aloe plants under different growth environments have significant statistical differences. The modified electrode was successfully applied to the dynamic change of aloe polysaccharide in aloe plants for real-time monitoring, which made the electrochemical detection method a promising method for monitoring the photosynthesis intensity of plants.

Key words: Carbonfiber microelectrode, Gold nanoparticles, Aloe polysaccharide, Dynamic detection

中图分类号:

O657.1

TrendMD:

王婷婷, 李元, 杨莉莉, 鲍昌昊, 程寒. 碳纤维微电极负载金纳米粒子用于芦荟多糖的在体动态监测. 高等学校化学学报, 2020, 41(1): 87.

WANG Tingting, LI Yuan, YANG Lili, BAO Changhao, CHENG Han. In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles ^†. Chem. J. Chinese Universities, 2020, 41(1): 87.

图/表 12

Fig.1 TEM image(A) and UV-Vis absorption spectrum(B) of AuNPs

Fig.2 SEM images of CFME(A) and AuNPs/CFME(B)

Fig.3 EDS image of AuNPs/CFME Inset: the red box in the SEM image of AuNPs/CFME is the detection window of EDS.

Fig.4 DPVs of aloe polysaccharide in water storage tissue gel layer of aloe leaves for AuNPs/CFME before(a) and after(b) adding 100 μL 10-2 mol/L glucose solution

Fig.5 Repetitive CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leaves with scanning speed of 0.1 V/s for CFME

Fig.6 CV curves of 1 mg/mL aloe polysaccharide in PBS(pH=7.0) with scanning speed of 0.1 V/s for AuNPs/CFME

Fig.7 Repetitive CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leave with scanning speed of 0.1 V/s for AuNPs/CFME

Fig.8 CV curves of aloe polysaccharide in aloe leaves water storage tissue gel layer for different electrodes

Fig.9 EIS curves of bare CFME(a), AuNPs/CFME(b) in 0.1 mol/L of KCl solution containing 5 mmol/L of [Fe(CN)6]3-/4-(A) and linear relationship between peak current and the square root of scanning rate for CFME(a) and AuNPs/CFME(b)(B) Inset of (A): Randles equivalence circuit model was used to fit the data.

Fig.10 CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leaves for AuNPs/CFME with different electrodeposition time(A) and effects of electrodeposition time on the oxidation peak current of aloe polysaccharide in water storage tissue gel layer of aloe leaves by CV measurements(B) Electrodeposition time/min: a. 10; b. 20; c. 30; d. 40.

Table 1 Electrochemical response of control group and experimental group(n=20)*

No.	I/nA		No.	I/nA
No.	Control group	Test group	No.	Control group	Test group
1	2.090	1.617	11	2.005	1.581
2	2.085	1.598	12	2.078	1.674
3	1.985	1.587	13	2.001	1.655
4	2.115	1.695	14	1.943	1.678
5	2.013	1.643	15	1.899	1.625
6	1.896	1.667	16	1.987	1.638
7	1.957	1.621	17	2.019	1.654
8	2.056	1.656	18	2.069	1.593
9	1.998	1.699	19	1.963	1.679
10	2.034	1.593	20	1.925	1.625

Fig.11 Histogram of 20 electrochemical responses of test group and control group in Table 1

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