高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (7): 1423.doi: 10.7503/cjcu20131268

• 有机化学 • 上一篇    下一篇

关白附中1,6-葡聚糖及其硫酸酯的抗补体活性

李晓军1,2, 蒋嘉烨1, 施松善2,3, 栗源1, 江永波1, 可燕1(), 王顺春2,3   

  1. 1. 上海中医药大学教学实验中心, 2. 中药研究所, 中药标准化教育部重点实验室, 3. 上海中药标准化研究中心, 上海 201203
  • 收稿日期:2013-12-24 出版日期:2014-07-10 发布日期:2014-04-29
  • 作者简介:联系人简介: 可 燕, 女, 博士, 教授, 主要从事中药活性成分筛选方面的研究. E-mail: keyantcm@163.com
  • 基金资助:
    上海市卫生局中医药科研基金(批准号: 2012J008A)和国家科学自然基金(批准号: 81373951)资助

Anti-complementary Activities of a (1→6) Linked Glucan from Korean Mondshood Root and Its Sulfated Derivatives

LI Xiaojun1,2, JIANG Jiaye1, SHI Songshan2,3, LI Yuan1, JIANG Yongbo1, KE Yan1,*(), WANG Shunchun2,3,*   

  1. 1. Teaching Experimental Center, 2. Key Laboratory for Standardization of Chinese Medicine of Ministry of Education, Institute of Chinese Materia Medica, 3. Shanghai R & D Center for Standardization of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2013-12-24 Online:2014-07-10 Published:2014-04-29
  • Contact: KE Yan,WANG Shunchun E-mail:keyantcm@163.com
  • Supported by:
    † Supported by the Traditional Chinese Medicine Scientific Research Foundation of Shanghai Health Bureau(No.2012J008A) and the Natio-nal Natural Science Foundation of China(No.81373951)

摘要:

为研究关白附多糖及其硫酸酯的经典途径抗补体活性, 以关白附[Aconitum coreanum(Lévl.) Raipaics]为原料, 经水提醇沉、 DE-32、 Superdex-200和Superdex-75凝胶柱分离纯化, 得到1个均一的中性多糖KMPS-2A. 采用高效凝胶渗透色谱法、 甲基化、 核磁共振和红外光谱等手段对KMPS-2A的结构进行了鉴定; 采用氯磺酸吡啶法制备了多糖硫酸酯, 并测定了多糖及硫酸酯的抗补体活性. 结果表明, KMPS-2A的平均相对分子量为6.76×105, 结构为α-1,6-D-Glc链接的线性多糖; 在氯磺酸与吡啶的体积比为1.75∶1.0时制备的多糖硫酸酯1.75B的取代度最高为1.79. 碳核磁谱分析结果表明, 硫酸基团先后取代C2, C3及C4位. 该多糖硫酸酯的抗补体活性与其硫酸基团取代度呈现一定的相关性, 多糖硫酸酯1.75B的经典途径抗补体活性优于阳性对照药肝素, 表明其具有开发成为补体抑制剂的潜力.

关键词: 多糖, 关白附, 补体

Abstract:

In order to study anti-complement activity in the classical pathway of Korean Mondshood root and its sulfated polysaccharides, a homogeneous polysaccharide(KMPS-2A) was gained from the Korean Mondshood root[Aconitum coreanum(Lévl.) Raipaics] by water extract and alcohol precipitate via DE-32, Superdex-200 and Superdex-75 gel filtration. Its structure was investigated by high performance gel permeation chromatography, methylation analysis, NMR and IR spectrometry. The results showed that the average molecular weights(Mw) of the KMPS-2A was 676000 and it was consisted of a repeating structure as [α-1,6-D-Glc]n. Sulfated derivatives were gained by chlorosulfonic acid-pyridine method and it was found that the sulfated derivatives had the highest degree of substitution(1.79) when the volume ratio of chlorosulfonic acid and pyridine was 1.75∶1.0. It was shown that sulfated polysaccharide 1.75B had a better anti-complementary activity. C2, C3 and C4 were substituted by sulfate groups. A certain correlation was presented between the degree of substitution and the anticomplementary activity of sulfated derivatives. Anticomplementary activity of sulfated derivative 1.75B was better than the heparin group in the classical pathway, which suggested the potential activity of the complement inhibitors.

Key words: Polysaccharide, Korean Mondshood root, Complement

中图分类号: 

TrendMD: