高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (11): 2430-2434.doi: 10.7503/cjcu20111169

• 分析化学 • 上一篇    下一篇

电喷雾质谱法研究防己诺林碱与G-四链体的相互作用

徐牛生1,2, 杨洪梅1,2, 崔勐1, 宋凤瑞1, 刘志强1, 刘淑莹1,3   

  1. 1. 中国科学院长春应用化学研究所, 长春 130022;
    2. 中国科学院研究生院, 北京 100049;
    3. 长春中医药大学人参研究院, 长春 130117
  • 收稿日期:2011-12-12 出版日期:2012-11-10 发布日期:2012-10-15
  • 通讯作者: 刘淑莹,女,博士,研究员,博士生导师,主要从事生物质谱学研究.E-mail:syliu19@yahoo.com.cn E-mail:syliu19@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 20873137, 21175127)和吉林省医药产业发展专项基金(批准号: YYZX201131)资助.

Investigation of Interaction of Fangchinoline with G-quadruplex DNA by Electrospray Ionization Mass Spectrometry

XU Niu-Sheng1,2, YANG Hong-Mei1,2, CUI Meng1, SONG Feng-Rui1, LIU Zhi-Qiang1, LIU Shu-Ying1,3   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China;
    3. Institute of Ginseng Research, Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2011-12-12 Online:2012-11-10 Published:2012-10-15

摘要:

采用电喷雾质谱法研究了防己诺林碱与双链核酸及G-四链体的相互作用. 结果表明, 防己诺林碱可选择性地与G-四链体结合. 利用串联质谱技术对防己诺林碱与核酸的结合模式进行了研究, 结果表明, 防己诺林碱可能通过末端堆积作用与G-四链体结合, 而通过插入作用与双链核酸结合. 结合模式的差异导致防己诺林碱选择性地与G-四链体结合.

关键词: 电喷雾质谱, 双链核酸, G-四链体, 防己诺林碱, 相互作用

Abstract:

Electrospray ionization mass spectrometry (ESI-MS) was used to investigate the interaction of fangchinoline with G-quadruplex and duplex DNA. The results showed that fangchinoline selectively bound to G-quadruplex DNA. Upon MS/MS experiments, complexes of fangchinoline and G-quadruplex DNA underwent the loss of fangchinoline, suggesting an end-stacking binding mode, while complexes of fangchinoline and duplex DNA underwent the loss of fangchinoline, suggesting the intercalated binding mode.

Key words: Electrospray ionization mass spectrometry(ESI-MS), Duplex DNA, G-quadruplex, Fangchinoline, Interaction

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