高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (02): 378-382.doi: 10.3969/j.issn.0251-0790.2012.02.029

• 物理化学 • 上一篇    下一篇

Gd3+与RGD共修饰量子点用于胰腺癌细胞的荧光及MR双模态成像

刘峰君1, 张兵波2, 宋歌1, 程英升1   

  1. 1. 同济大学附属第十人民医院影像临床医学中心, 上海 200072;
    2. 同济大学先进材料与纳米生物医学研究院, 上海 200092
  • 收稿日期:2011-03-11 出版日期:2012-02-10 发布日期:2012-01-13
  • 通讯作者: 张兵波, 男, 博士, 副研究员, 主要从事生物医学材料及其应用研究. E-mail: bingbozhang@tongji.edu.cn 程英升, 男, 博士, 教授, 博士生导师, 主要从事医学影像研究. E-mail: cjr.chengysh@vip.163.com E-mail:bingbozhang@tongji.edu.cn;cjr.chengysh@vip.163.com
  • 基金资助:

    国家自然科学基金(批准号: 51003078)、上海市科委基础研究重点项目(批准号: 10JC1412900)、同济大学青年优秀人才培养计划项目(批准号: 2009KJ072)和苏州大学现代丝绸国家工程实验室基金资助.

Gd3+ and RGD Functionalized Quantum Dots for Fluorescent and MR Dual-modality Imaging of Pancreatic Cancer Cells

LIU Feng-Jun1, ZHANG Bing-Bo2, SONG Ge1, CHENG Ying-Sheng1   

  1. 1. Medical Imaging Center, the Tenth People's Hospital of Tongji University, Shanghai 200072, China;
    2. Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092, China
  • Received:2011-03-11 Online:2012-02-10 Published:2012-01-13
  • Contact: Bo BingZHANG E-mail:bingbozhang@tongji.edu.cn;cjr.chengysh@vip.163.com

摘要: 结合磁共振成像(MRI)和荧光成像技术, 以钆离子(Gd3+)、量子点及精氨酸(R)-甘氨酸(G)-天冬氨酸(D)(RGD)多肽等为功能单元, 采用纳米载体组装技术构建了MRI弛豫率/荧光效率高和靶向性强的Gd3+与RGD共修饰的量子点双模态纳米探针(QDs@Gd3+-RGD), 并将其用于胰腺癌细胞的双模态成像. 实验结果表明, QDs@Gd3+-RGD双模态纳米探针具有较高的弛豫率, 且能对胰腺癌patu8988细胞进行荧光和T1-weighted MR成像.

关键词: 双模态成像, 量子点, RGD, 胰腺癌

Abstract: Though early diagnosis of pancreatic cancer is difficult, it brings challenges and opportunities to the molecular imaging. The existing diagnostic imaging technologies are limited as a result of single functiona-lity, making it difficult for accurate diagnosis. In this work, magnetic resonance image(MRI) and fluorescence imaging were combined by several key contrast agents including gadolinium ion, quantum dots(QDs) and arginine(R)-glycine(G)-D-aspartic acid(D)(RGD) for high relaxation rate, strong fluorescent emission and good targeting. The integrated dual-modality QDs@Gd3+-RGD nanoprobes were used for dual-modality imaging of pancreatic cancer cells. TEM and dynamic light scattering(DLS) results indicate complete dispersion of QDs@Gd3+-RGD nanoprobes in water. In vitro relaxivity measurement shows high relaxation rate of QDs@Gd3+-RGD nanoprobes. Their r1 and r2 are 7.6 and 9.5 L@mmol-1@s-1, respectively. Fluorescent and MR dual-modality imaging suggest the prepared QDs@Gd3+-RGD nanoprobes can sever as both fluorescent and MR imaging of patu8988 pancreatic cancer cells.

Key words: Dual-modality imaging, Quantum dots, Arginine(R)-glycine(G)-D-aspartic acid(D)(RGD), Pancreatic cancer

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