高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (01): 44.doi: 10.3969/j.issn.0251-0790.2012.01.007

• 分析化学 • 上一篇    下一篇

基于核苷类化合物HILIC-ESI-TOF/MS分析的海洋动物药指纹图谱方法

赵恒强1,2, 陈军辉2, 史倩2, 李鑫2, 曹为2, 李景喜2, 王小如2,3   

  1. 1. 中国海洋大学化学化工学院, 青岛 266100;
    2. 国家海洋局第一海洋研究所, 青岛市现代分析技术及中药标准化重点实验室, 青岛 266061;
    3. 厦门大学化学化工学院, 厦门 361005
  • 收稿日期:2011-01-18 出版日期:2012-01-10 发布日期:2011-12-20
  • 通讯作者: 陈军辉,男,博士,助理研究员,主要从事天然药物现代化研究.E-mail:jhchen@fio.org.cn E-mail:jhchen@fio.org.cn
  • 基金资助:

    国家自然科学基金(批准号: 20905017), 国家"八六三"计划项目(批准号: 2007AA092001-10), 国家海洋局青年基金(批准号: 2010140)和国家海洋局第一海洋研究所基本科研业务专项(批准号: 2010G25)资助.

New Fingerprinting Method Based on the Distribution of Nucleoside Compounds for Marine Animal Drugs by HILIC-ESI-TOF/MS

ZHAO Heng-Qiang1,2, CHEN Jun-Hui2, SHI Qian2, LI Xin2, CAO Wei2, LI Jing-Xi2, WANG Xiao-Ru2,3   

  1. 1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China;
    2. Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;
    3. Department of Chemistry of College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2011-01-18 Online:2012-01-10 Published:2011-12-20
  • Contact: CHEN Jun-Hui E-mail:jhchen@fio.org.cn

PDF (PC)

被引次数

15

摘要： 探讨了核苷类化合物指纹图谱用于不同海洋动物药真伪鉴别的可行性, 为贵重动物药的鉴别提供了一种新方法. 采用亲水色谱-电喷雾飞行时间质谱(HILIC-ESI-TOF/MS)对不同海洋动物药中的16种核苷类化合物进行分析, 构建了基于16种核苷类化合物的特征指纹图谱, 结合相似度分析和聚类分析, 用于不同海洋动物药的鉴别. 结果表明, 基于核苷类化合物HILIC-ESI-TOF/MS分析的指纹图谱能反映不同海洋动物药各自的固有特征, 结合相似度分析和聚类分析可实现对不同海洋动物药的正确区分. 说明核苷类化合物指纹图谱有望成为动物药鉴别的新方法.

关键词: 核苷类化合物, 动物药, 指纹图谱, 亲水色谱, 飞行时间质谱

Abstract: The feasibility of identification of the authenticity for various marine animal drugs was investigated by a fingerprinting method based on the distribution of nucleoside compounds, and a new method for the discrimination of precious animal drugs was provided. Sixteen nucleoside compounds in various marine animal drugs were determined by hydrophilic interaction liguid chromatography-electrospray ionization-time of flight-mass spectrometry(HILIC-ESI-TOF/MS). Characteristic fingerprint based on the distribution of 16 nucleosides was constructed, and it was used for identification of the authenticity of various marine animal drugs combined with similarity analysis and clustering analysis. The results indicated that the proposed characteristic fingerprint could reflect the inherent characteristics of their own for various marine animal drugs. Furthermore, it could be used to distinguish different marine animal drugs combined with similarity analysis and clustering analysis. In conclusion, the developed fingerprinting method is expected to be a powerful tool for the identification of animal drugs.

Key words: Nucleoside, Animal drug, Fingerprint, Hydrophilic interaction liquid chromatography(HILIC), Time of flight-mass spectrometry(TOF/MS)

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