Glycol-unit Structure Identification of a Kind of Exopolysaccharide from a Lactic Acid Bacterium

Chem. J. Chinese Universities

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Glycol-unit Structure Identification of a Kind of Exopolysaccharide from a Lactic Acid Bacterium

LI Quan-Yang^1*, XIA Wen-Shui², XU De-Ping²

1. College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, China;
2. School of Food Science, Southern Yangtze University, Wuxi 214036, China

Received:2006-05-29 Revised:1900-01-01 Online:2007-04-10 Published:2007-04-10
Contact: LI Quan-Yang

Abstract

Abstract: The structure of exopolysaccharide EPS03-1gm was identified by the technology of GC, UV spectrophotometric analysis and NMR. The EPS03-1gm was separated and purified before analysis which was secreted from S. thermophilus. The results show that the mono-glycos of EPS03-1gm were glucose and galactose and there are no glycol-aldehudic acid or protein ingredient. The ¹H NMR made it clear that there were four glycol-cells in each repeat glycol-unit. The DEPT reveals there were four CH₂ in every repeat glycol-unit. The positions of H were confirmed by analyzing the TOCSY spectrum. The positions of H and the order of glycol-cell were confirmed by analyzing the HMQC and HMBC spectra. Finally, the glycol-unit structure were fixed on as follows: →3)-α-D-Galp-(1→3)-β-D-Galf-(1→3)-β-D-Glcp-(1→6)-β-D-Glcp-(1→.

Key words: Exopolysacharides of lactic acid bacteria, NMR, Structure identification

CLC Number:

O629.12

TrendMD:

LI Quan-Yang^1*, XIA Wen-Shui², XU De-Ping². Glycol-unit Structure Identification of a Kind of Exopolysaccharide from a Lactic Acid Bacterium[J]. Chem. J. Chinese Universities, doi: .

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Glycol-unit Structure Identification of a Kind of Exopolysaccharide from a Lactic Acid Bacterium

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