Design and Synthesis of a Novel Fluorescein-based Fluorescent Probe for Labeling of Histidine in Human Serum

Abstract

Abstract: The synthesis of a novel long-wavelength fluorescent probe, 3-epoxypropoxy fluorescein, and its properties for labeling of histidine are briefly described in this communication.The probe is highly selective for histidine, and other amino acids with a concentration of 1000 times higher than that of histidine did not show noticeable interferences.As an application of this probe, fluorescent labeling of histidine in human serum was attempted and the obtained results were in agreement with those given by using histidine-nickel complex adsorptive voltammetry, both of which were within the normal range of the results reported in literatures.

Key words: Fluorescent probe, Histidine labeling, Fluorescein derivative, Epoxy labeling group

CLC Number:

O657

TrendMD:

LI Xiao-Hua, MA Hui-Min, DONG Su-Ying, DUAN Xue-Jun, NIE Li-Hua, SUN Ming . Design and Synthesis of a Novel Fluorescein-based Fluorescent Probe for Labeling of Histidine in Human Serum[J]. Chem. J. Chinese Universities, 2003, 24(11): 1984.

References

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