Cytosine-rich Oligonucleotide-templated Fluorescent Silver Nanoclusters for Sensitive Assay of S1 Nuclease

doi:10.7503/cjcu20170113

Abstract

Abstract:

A label-free fluorescence method for detection of S1 nuclease was developed using oligonucleotide-templated silver nanoclusters(AgNCs). Cytosine-rich single strand DNA(C-rich ssDNA) scaffolded silver nanoclusters were devised and synthesized as a functional probe. S1 nuclease can specifically recognize ssDNA, which is degraded into mononucleotide and oligonucleotide fragment under the optimum enzyme-catalyzed reaction condition. In the absence of S1 nuclease, the silver nanoclusters could be effectively synthesized by C-rich ssDNA, which produced obvious fluorescence intensity. In the presence of S1 nuclease, C-rich ssDNA probes were specifically recognized and effectively digested into small fragments. As a result, the silver nanoclusters synthesis was suspended leading a fluorescence reduction. A good linear correlation was obtained between fluorescence change and the logarithm of the Cu²⁺ ions concentration over the range from 5.0×10^-5 U/μL to 4.0×10^-3 U/μL. The detection limit was estimated as 2.0×10^-6 U/μL. The proposed method was highly selective and there were no obvious interfering effects on the determination from Bst polymerase, Thrombin, ExoⅢ, E.coli ligase and BSA. The proposed sensor reveals good recovery rates from 91.8% to 109.5% in RPMI 1640 cell medium, indicating that the sensing system was feasible for the detection of S1 nuclease in practical samples. This label-free, simple and cost-effective strategy has a potential application in biomedical research and clinical early diagnosis.

Key words: Silver nanoclusters, Cytosine-rich ssDNA, S1 nuclease, Fluorescent probe

CLC Number:

O657.3

TrendMD:

WANG Xingxing, LI Panpan, HE Jinglin, OUYANG Wen, XIAO Hui, YANG Chan, CAO Zhong. Cytosine-rich Oligonucleotide-templated Fluorescent Silver Nanoclusters for Sensitive Assay of S1 Nuclease[J]. Chem. J. Chinese Universities, 2017, 38(8): 1334.

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