Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand

doi:10.3969/j.issn.0251-0790.2012.03.007

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (03): 470.doi: 10.3969/j.issn.0251-0790.2012.03.007

• Analytical Chemistry • Previous Articles Next Articles

Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand

QIU Chang-Gui¹, WANG Xiao-Yu¹, GUO Zhen-Peng¹, SONG Li-Juan¹, CHEN Yi^1,2,3

1. Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2. Beijing National Laboratory for Molecular Science, Beijing 100190, China;
3. Beijing Center of Mass Spectrometry, Beijing 100190, China

Received:2011-04-12 Online:2012-03-10 Published:2012-03-10

Abstract

Abstract:

Chiral ligand-exchange capillary electrophoresis is flexible but can not be applied to the analysis of unknown samples or chiral compounds without a chiral ligand. We tried to solve this problem by exploring some non-analyte chiral ligands aiming at the assaying the L-dopa tablets. With aromatic D,L-amino acids (dopa analogues) as mimetic analytes, chiral selecting systems of Cu²⁺-tartarate and Ni²⁺-tartarate were obtained among the metal complexes of Cu²⁺, Ni²⁺, Zn²⁺ and Co²⁺ formed from tartaric, lactic and malic acids. Cu²⁺-tartarate worked better at an acidic condition while Ni²⁺-tartarate better at a weakly basic condition. The latter system suited for quantitative analysis of L- and D-dopa in oral tablets. Its linear range was in between 0.025-2.5 mmol/L D- or L-dopa, with linear coefficients all above 0.9999 and recovery at around 96.3%. The detection limit reached about 0.5%(mass fraction) D-dopa. Several batches of L-dopa oral tablets were then assayed, given the content of (97.33?0.70)%(mass fraction) of the specified data. The ratio of ligand to central ion was found to be the most crucial factor, besides, pH and complex concentration were also highly important. Other conditions such as additives and the type of buffer reagents impacted also obviously on the chiral resolution and better optimized.

Key words: Ligand-exchange capillary electrophoresis, Metal-hydroxyl acid complex, Chiral separation, Amino acid, Dopa assay

CLC Number:

O657.8

TrendMD:

QIU Chang-Gui, WANG Xiao-Yu, GUO Zhen-Peng, SONG Li-Juan, CHEN Yi. Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand[J]. Chem. J. Chinese Universities, 2012, 33(03): 470.

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Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand

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